RGD Reference Report - Detection of prolactin receptor mRNA in the corpus striatum and substantia nigra of the rat. - Rat Genome Database

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Detection of prolactin receptor mRNA in the corpus striatum and substantia nigra of the rat.

Authors: Pi, X  Voogt, JL  Grattan, DR 
Citation: Pi X, etal., J Neurosci Res 2002 Feb 15;67(4):551-8.
RGD ID: 729660
Pubmed: PMID:11835322   (View Abstract at PubMed)
DOI: DOI:10.1002/jnr.10147   (Journal Full-text)

The observation of prolactin modulation of the nigrostriatal dopaminergic system suggests the expression of prolactin receptor in the corpus striatum or substantia nigra. The present study investigated expression of prolactin receptor mRNA in tissues microdissected from the corpus striatum and substantia nigra of the rat. By using reverse transcription PCR combined with Southern hybridization, the long form of prolactin receptor mRNA was detected in the substantia nigra, caudate putamen, globus pallidus, and ventral pallidum in ovariectomized rats, whereas the short form was not detectable in any of these areas. Estrogen had no effect on expression of the long-form mRNA in the substantia nigra and corpus striatum. By using the RNase protection assay, the expression of both short and long forms of prolactin receptor mRNA was observed in the corpus striatum in ovariectomized rats. Again, levels of expression were not significantly altered by estrogen treatment. Both forms of prolactin receptor mRNA were clearly expressed in the choroid plexus and were up-regulated by estrogen treatment. The expression of both forms of prolactin receptor mRNA in nigrostriatal areas may help to support the hypothesis that prolactin has direct actions on these brain regions.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
prolactin receptor activity  IMP 729660 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prlr  (prolactin receptor)


Additional Information