RGD Reference Report - Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor alpha mRNA, glucocorticoid receptor beta mRNA and P300/CBP protein in lupus nephritis mice.

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Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor alpha mRNA, glucocorticoid receptor beta mRNA and P300/CBP protein in lupus nephritis mice.
Authors:	Wu, XL Zhang, WG Shi, XM An, P Sun, WS Qiao, CL Wang, Z
Citation:	Wu XL, etal., Chin J Integr Med. 2011 Apr;17(4):277-82. Epub 2011 Apr 21.
RGD ID:	7174713
Pubmed:	PMID:21509671 (View Abstract at PubMed)
DOI:	DOI:10.1007/s11655-011-0693-3 (Journal Full-text)
OBJECTIVE: To investigate the therapeutic effects and mechanisms of using artemisinin (Art) combined with glucocorticoid (GC) to treat lupus nephritis (LN) mice. METHODS: Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table: control group, model group, prednisone group administrated with 6.45 mg/(kg.d) prednisone suspension, and Art+prednisone group administrated with 150 mg/(kg.d) Art suspension and 3.225 mg/(kg.d) prednisone suspension. A mice model of LN was established by injection with living lymph cell suspension. The changes of urine protein/24h, the expressions of GC receptor alpha (GRalpha) mRNA, GC receptor beta (GRbeta) mRNA in peripheral blood mononuclear cells (PBMCs), and transcriptional coactivator P300/CBP protein in renal tissue were measured. RESULTS: Compared with the model group, the treatment groups had significant decrease in urine protein/24 h, and renal pathological lesion (P<0.01). In the same groups, the expression of transcriptional coactivator P300/CBP protein in renal tissue and GRalpha mRNA were significantly increased, and GRbeta mRNA expression was significantly decreased (P<0.01). And the Art+prednisone group has a better therapeutic effect than the prednisone group (P<0.01). CONCLUSIONS: Art has therapeutic sensitization effects on GC in the LN mice. The underlying mechanism could be correlated with the effect of Art on the increase of the expressions of GRalpha mRNA and transcriptional coactivator P300 300/CBP protein in renal tissue and on the decrease of the expression of GRbeta mRNA in PBMC.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
NR3C1	Human	lupus nephritis		ISO	Nr3c1 (Mus musculus)		RGD
Nr3c1	Rat	lupus nephritis		ISO	Nr3c1 (Mus musculus)		RGD
Nr3c1	Mouse	lupus nephritis		IEP			RGD

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Genes (Rattus norvegicus)

Nr3c1 (nuclear receptor subfamily 3, group C, member 1)

Genes (Mus musculus)

Nr3c1 (nuclear receptor subfamily 3, group C, member 1)

Genes (Homo sapiens)

NR3C1 (nuclear receptor subfamily 3 group C member 1)

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Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor alpha mRNA, glucocorticoid receptor beta mRNA and P300/CBP protein in lupus nephritis mice.