RGD Reference Report - [Nuclear factor kappa B impairs insulin signaling pathway in skeletal muscle cells of rat with sepsis]. - Rat Genome Database

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[Nuclear factor kappa B impairs insulin signaling pathway in skeletal muscle cells of rat with sepsis].

Authors: Yan, Xiao-wen  Li, Wei-qin  Li, Qiu-rong  Li, Ning  Li, Jie-shou 
Citation: Yan XW, etal., Zhonghua Wai Ke Za Zhi. 2009 Aug 15;47(16):1257-60.
RGD ID: 6482862
Pubmed: PMID:19781177   (View Abstract at PubMed)


OBJECTIVE: To investigate the effects of nuclear factor kappa B (NF-kappaB) on insulin signaling in skeletal muscle cells of rat with sepsis.
METHODS: SD rats were randomly divided into two groups: control group and sepsis group.Sepsis model was reproduced by cecal ligation and puncture in sepsis group. At 8, 16, 24, 48 and 72 h after operation, the gastrocnemius was harvested. Conventional HE staining was used to observe the morphology of skeletal muscle cells. IRS-1 protein and tyrosine phosphorylation of IRS-1 and Ser(307) phosphorylation of IRS-1 were detected by Western Blotting and immuno-precipitation. Activities of NF-kappaB in skeletal muscle cells were detected by electrophoretic mobility shift assay.
RESULTS: Tyrosine phosphorylation of IRS-1 in sepsis group was significantly lower than in control group (P < 0.01), while Ser(307) phosphorylation of IRS-1 in sepsis group was significantly higher than in control group (P < 0.01). In sepsis group, NF-kappaB activity in skeletal muscle cells was significantly higher than in control group (P < 0.01). There was significant negative correlation between activity of NF-kappaB and tyrosine phosphorylation of IRS-1 (r = 0.972, P < 0.01). There was significant positive correlation between activities of NF-kappaB and Ser(307) phosphorylation of IRS-1 (r = 0.969, P < 0.01).
CONCLUSIONS: There is no inflammatory cell infiltrate in skeletal muscle cells with sepsis. But the activity of NF-kappaB in skeletal muscle cells is obviously enhanced, and it is closely related with disorder of insulin signaling in skeletal muscle cells of rat with sepsis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IRS1HumanSepsis  ISOIrs1 (Rattus norvegicus)protein:decreased tyrosine phosphorylation and increased serine phosphorylation:gastrocnemiusRGD 
Irs1RatSepsis  IDA protein:decreased tyrosine phosphorylation and increased serine phosphorylation:gastrocnemiusRGD 
Irs1MouseSepsis  ISOIrs1 (Rattus norvegicus)protein:decreased tyrosine phosphorylation and increased serine phosphorylation:gastrocnemiusRGD 

Objects Annotated

Genes (Rattus norvegicus)
Irs1  (insulin receptor substrate 1)

Genes (Mus musculus)
Irs1  (insulin receptor substrate 1)

Genes (Homo sapiens)
IRS1  (insulin receptor substrate 1)


Additional Information