RGD Reference Report - [Nuclear factor kappa B impairs insulin signaling pathway in skeletal muscle cells of rat with sepsis]. - Rat Genome Database

RGD Reference Report - [Nuclear factor kappa B impairs insulin signaling pathway in skeletal muscle cells of rat with sepsis]. - Rat Genome Database

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[Nuclear factor kappa B impairs insulin signaling pathway in skeletal muscle cells of rat with sepsis].
Authors:	Yan, Xiao-wen Li, Wei-qin Li, Qiu-rong Li, Ning Li, Jie-shou
Citation:	Yan XW, etal., Zhonghua Wai Ke Za Zhi. 2009 Aug 15;47(16):1257-60.
RGD ID:	6482862
Pubmed:	PMID:19781177 (View Abstract at PubMed)
OBJECTIVE: To investigate the effects of nuclear factor kappa B (NF-kappaB) on insulin signaling in skeletal muscle cells of rat with sepsis. METHODS: SD rats were randomly divided into two groups: control group and sepsis group.Sepsis model was reproduced by cecal ligation and puncture in sepsis group. At 8, 16, 24, 48 and 72 h after operation, the gastrocnemius was harvested. Conventional HE staining was used to observe the morphology of skeletal muscle cells. IRS-1 protein and tyrosine phosphorylation of IRS-1 and Ser(307) phosphorylation of IRS-1 were detected by Western Blotting and immuno-precipitation. Activities of NF-kappaB in skeletal muscle cells were detected by electrophoretic mobility shift assay. RESULTS: Tyrosine phosphorylation of IRS-1 in sepsis group was significantly lower than in control group (P < 0.01), while Ser(307) phosphorylation of IRS-1 in sepsis group was significantly higher than in control group (P < 0.01). In sepsis group, NF-kappaB activity in skeletal muscle cells was significantly higher than in control group (P < 0.01). There was significant negative correlation between activity of NF-kappaB and tyrosine phosphorylation of IRS-1 (r = 0.972, P < 0.01). There was significant positive correlation between activities of NF-kappaB and Ser(307) phosphorylation of IRS-1 (r = 0.969, P < 0.01). CONCLUSIONS: There is no inflammatory cell infiltrate in skeletal muscle cells with sepsis. But the activity of NF-kappaB in skeletal muscle cells is obviously enhanced, and it is closely related with disorder of insulin signaling in skeletal muscle cells of rat with sepsis.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
IRS1	Human	Sepsis		ISO	Irs1 (Rattus norvegicus)	protein:decreased tyrosine phosphorylation and increased serine phosphorylation:gastrocnemius	RGD
Irs1	Rat	Sepsis		IDA		protein:decreased tyrosine phosphorylation and increased serine phosphorylation:gastrocnemius	RGD
Irs1	Mouse	Sepsis		ISO	Irs1 (Rattus norvegicus)	protein:decreased tyrosine phosphorylation and increased serine phosphorylation:gastrocnemius	RGD

Genes (Rattus norvegicus)


Irs1 (insulin receptor substrate 1)

Genes (Mus musculus)


Irs1 (insulin receptor substrate 1)

Genes (Homo sapiens)


IRS1 (insulin receptor substrate 1)