RGD Reference Report - Discrepancy between serum levels of low molecular weight proteins in acute kidney injury model rats with bilateral ureteral obstruction and bilateral nephrectomy. - Rat Genome Database

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Discrepancy between serum levels of low molecular weight proteins in acute kidney injury model rats with bilateral ureteral obstruction and bilateral nephrectomy.

Authors: Tsuda, H  Isaka, Y  Takahara, S  Horio, M 
Citation: Tsuda H, etal., Clin Exp Nephrol. 2009 Dec;13(6):567-70. Epub 2009 Jun 18.
RGD ID: 6482685
Pubmed: PMID:19536607   (View Abstract at PubMed)
DOI: DOI:10.1007/s10157-009-0203-5   (Journal Full-text)

BACKGROUND: Serum levels of low molecular weight (LMW) proteins, such as cystatin C and beta-2-microglobulin (MG), are used as GFR markers. Ureteral obstruction is a cause of acute kidney injury (AKI). However, it is unclear whether serum concentrations of LMW proteins are increased in postrenal AKI like in intrinsic AKI. To evaluate this question, the responses of serum levels of cystatin C and beta-2-MG were investigated in rats with bilateral ureteral obstruction (BUO) and bilateral nephrectomy (BNX). METHODS: Sixteen SD rats were divided into sham (sham operated), BUO and BNX groups. Serum levels of creatinine, BUN, cystatin C, beta 2-MG and creatine phosphokinase (CPK) were measured after 24 h of the treatments. Cystatin C and beta 2-MG were measured using rat ELISA kits. RESULTS: In the sham group, the mean +/- SD of creatinine, BUN, cystatin C and beta-2-MG were 0.19 +/- 0.01 mg/dl, 22.6 +/- 1.9 mg/dl, 1.27 +/- 0.18 mg/l and 3.57 +/- 0.42 mg/l, respectively. Following BNX, the values significantly rose to 4.79 +/- 0.17 mg/dl, 116.9 +/- 2.6 mg/dl, 2.59 +/- 0.18 mg/l and 9.03 +/- 0.52 mg/l compared with sham, respectively. Following BUO, they were 3.99 +/- 0.25 mg/dl, 130.3 +/- 12.9 mg/dl, 1.94 +/- 0.35 mg/l and 4.78 +/- 0.24 mg/l, respectively. Increasing amounts of creatinine and BUN in BUO were similar to those in BNX. On the other hand, cystatin C and beta-2 MG in BUO were much lower compared with BNX (P < 0.01 and <0.001, respectively). Fold increases of cystatin C and beta-2-MG in BUO were only 51% and 22% of those in BNX, respectively. There was no significant difference in CPK levels between the BUO and BNX groups. CONCLUSION: We showed a discrepancy between serum levels of LMW proteins in AKI model rats with BUO and BNX. The reasons for the discrepancy are discussed.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
acute kidney failure  ISOB2m (Rattus norvegicus)6482685; 6482685protein:increased expression:serumRGD 
acute kidney failure  IEP 6482685protein:increased expression:serumRGD 
ureteral obstruction  ISOB2m (Rattus norvegicus)6482685; 6482685protein:increased expression:serumRGD 
ureteral obstruction  IEP 6482685protein:increased expression:serumRGD 

Objects Annotated

Genes (Rattus norvegicus)
B2m  (beta-2 microglobulin)

Genes (Mus musculus)
B2m  (beta-2 microglobulin)

Genes (Homo sapiens)
B2M  (beta-2-microglobulin)


Additional Information