RGD Reference Report - Phosphorylation of c-Cbl protooncogene product following ethanol administration in rat cerebellum: possible involvement of Fyn kinase. - Rat Genome Database

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Phosphorylation of c-Cbl protooncogene product following ethanol administration in rat cerebellum: possible involvement of Fyn kinase.

Authors: Nishio, H  Otsuka, M  Kinoshita, S  Tokuoka, T  Nakajima, M  Noda, Y  Fukuyama, Y  Suzuki, K 
Citation: Nishio H, etal., Brain Res 2002 Sep 20;950(1-2):203-9.
RGD ID: 632732
Pubmed: PMID:12231245   (View Abstract at PubMed)

We have previously shown that ethanol administration results in tyrosine phosphorylation of the 130 kDa protein in rat brain, and identified the protein as Cas, the crk-associated src substrate. In the present study, we demonstrate that Cbl of a 120 kDa protein is also tyrosine-phosphorylated in the cerebellum in response to ethanol administration. We also investigated whether Fyn kinase was involved in ethanol-induced Cbl phosphorylation. Immunoprecipitation experiments showed that the amount of coimmunoprecipitated Fyn kinase with an anti-Cbl antibody increased in extracts from ethanol-administered rats compared to those from saline-administered rats. Exogenous Fyn kinase was shown to phosphorylate on tyrosine residue(s) of Cbl from the cerebellum in vitro. Furthermore, Fyn kinase and Cbl were demonstrated immunohistochemically to be coexpressed in white matter in the cerebellum. These findings indicate that Cbl is tyrosine-phosphorylated in rat cerebellum in response to ethanol administration, and also raise the possibility that Fyn kinase may be involved in the process.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FynRatpeptidyl-tyrosine phosphorylation  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FynRatenzyme binding  IPICbl (Rattus norvegicus) RGD 
FynRatprotein tyrosine kinase activity  IDA  RGD 
CblRatprotein tyrosine kinase binding  IPIFyn (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cbl  (Cbl proto-oncogene)
Fyn  (FYN proto-oncogene, Src family tyrosine kinase)

Objects referenced in this article
Gene Cblb Cbl proto-oncogene B Rattus norvegicus

Additional Information