RGD Reference Report - Short term hypothyroidism affects ovarian function in the cycling rat. - Rat Genome Database

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Short term hypothyroidism affects ovarian function in the cycling rat.

Authors: Hapon, MB  Gamarra-Luques, C  Jahn, GA 
Citation: Hapon MB, etal., Reprod Biol Endocrinol. 2010 Feb 11;8:14.
RGD ID: 4890368
Pubmed: PMID:20149258   (View Abstract at PubMed)
PMCID: PMC2841189   (View Article at PubMed Central)
DOI: DOI:10.1186/1477-7827-8-14   (Journal Full-text)

BACKGROUND: Rats made hypothyroid with propilthyouracil start showing abnormal cycling on the second cycle after the start of the treatment, with a high proportion of spontaneous pseudopregnancies and reduced fertility. METHODS: To investigate some of the mechanisms involved in these reproductive abnormalities, hypothyroidism was induced in virgin rats by propilthyouracil (0.1 g/L in the drinking water) and we determined circulating hormones by radioimmunoassay and whole ovary expression of ovarian hormone receptors, growth factors and steroidogenic enzymes using semi-quantitative RT-PCR.The study was performed on days 6 to 9 of treatment, corresponding to diestrus I (at 20.00-22.00 h), diestrus II (at 20.00-22.00 h), proestrus and estrus (both at 8.00-10.00 h and 20.00-22.00 h) of the second estrous cycle after beginning propilthyouracil treatment. Another group of rats was mated on day 8 and the treatment continued through the entire pregnancy to evaluate reproductive performance. RESULTS: Hypothyroidism increased circulating prolactin and estradiol on estrus 5 to 7-fold and 1.2 to 1.4-fold respectively. Growth hormone and insulin-like growth factor 1 diminished 60 and 20% respectively on proestrus morning. Hypothyroidism doubled the ovarian mRNA contents of estrogen receptor-beta on proestrus and estrus evenings, cyp19A1 aromatase mRNA on estrus evening and of growth hormone receptor on proestrus evening. Hypothyroidism did not influence ovulation rate or the number of corpora lutea at term, but a diminished number of implantation sites and pups per litter were observed (Hypothyroid: 11.7 +/- 0.8 vs. Control: 13.9 +/- 0.7). CONCLUSIONS: Short term hypothyroidism alters normal hormone profile in the cycling rat increasing the expression of estrogen receptor-beta and cyp19A1 aromatase on estrus, which in turn may stimulate estradiol and prolactin secretion, favouring corpus luteum survival and the subsequent instauration of pseudopregnancy.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CYP19A1Humanhypothyroidism  ISOCyp19a1 (Rattus norvegicus)mRNA:increased expression:ovary (rat)RGD 
Cyp19a1Rathypothyroidism  IEP mRNA:increased expression:ovary (rat)RGD 
Cyp19a1Mousehypothyroidism  ISOCyp19a1 (Rattus norvegicus)mRNA:increased expression:ovary (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp19a1  (cytochrome P450, family 19, subfamily a, polypeptide 1)

Genes (Mus musculus)
Cyp19a1  (cytochrome P450, family 19, subfamily a, polypeptide 1)

Genes (Homo sapiens)
CYP19A1  (cytochrome P450 family 19 subfamily A member 1)


Additional Information