RGD Reference Report - Participation of WD repeat-containing protein 54 (WDR54) in rat sperm-oocyte fusion through interaction with both IZUMO1 and JUNO. - Rat Genome Database

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Participation of WD repeat-containing protein 54 (WDR54) in rat sperm-oocyte fusion through interaction with both IZUMO1 and JUNO.

Authors: Lai, Xiong  Liu, Ruizhuo  Li, Mengyu  Fan, Yaochun  Li, Hongxia  Han, Guotao  Guo, Ruijie  Ma, Hairui  Su, Huimin  Xing, Wanjin 
Citation: Lai X, etal., Theriogenology. 2024 Jan 15;214:286-297. doi: 10.1016/j.theriogenology.2023.10.031. Epub 2023 Nov 7.
RGD ID: 408345208
Pubmed: PMID:37951137   (View Abstract at PubMed)
DOI: DOI:10.1016/j.theriogenology.2023.10.031   (Journal Full-text)

Fertilization is a complex process that depends on the fusion of the cell membrane of sperm with that of oocyte, and it involves sperm-oocyte recognition, binding, and fusion, which are mediated by multiple proteins. Among those proteins, IZUMO1 and its receptor JUNO have been identified as essential factors for sperm-oocyte recognition and fusion. However, the interaction between IZUMO1 and JUNO alone does not lead to cell membrane fusion, suggesting the involvement of additional proteins in sperm-oocyte membrane fusion. In this study, we have discovered that a protein called WDR54, which consists of WD-repeat modules, is located on the cell membrane of sperm, as well as on the cell membrane and in the cytoplasm of the oocyte. We have found that WDR54 is involved in sperm-oocyte fertilization. When sperm and oocyte were treated with anti-WDR54 ascites, the in vitro fertilization (IVF) rate significantly decreased. Furthermore, our research has shown that WDR54 interacts with both IZUMO1 and JUNO, and it colocalizes with IZUMO1 on the surface of the sperm head and with JUNO on the oocyte surface. Through structural analysis of the putative complexes of WDR54-IZUMO1 and WDR54-JUNO, we infer that these three proteins could form a complex of JUNO-WDR54-IZUMO1-JUNO (referred to as the "JWIJ complex") on the oocyte surface. Our findings suggest that WDR54 is an important factor involved in sperm-oocyte adhesion and fusion. This discovery provides new insight into the mechanisms of mammalian sperm-oocyte adhesion and fusion.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Wdr54Ratfusion of sperm to egg plasma membrane involved in single fertilization involved_inIMP PMID:37951137UniProt 
Wdr54Ratsingle fertilization involved_inIMP PMID:37951137UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Wdr54Ratcytoplasm located_inIDA PMID:37951137UniProt 
Izumo1Ratplasma membrane located_inIDA PMID:37951137UniProt 
Izumo1rRatplasma membrane located_inIDA PMID:37951137UniProt 
Wdr54Ratplasma membrane located_inIDA PMID:37951137UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Izumo1Ratprotein binding enablesIPIUniProtKB:D3ZX63PMID:37951137UniProt 
Izumo1rRatprotein binding enablesIPIUniProtKB:D3ZX63PMID:37951137UniProt 
Wdr54Ratprotein binding enablesIPIUniProtKB:F1M928PMID:37951137UniProt 
Wdr54Ratprotein binding enablesIPIUniProtKB:Q6AY06PMID:37951137UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Izumo1  (izumo sperm-oocyte fusion 1)
Izumo1r  (IZUMO1 receptor, JUNO)
Wdr54  (WD repeat domain 54)


Additional Information