RGD Reference Report - MiR-101a-3p Attenuated Pilocarpine-Induced Epilepsy by Downregulating c-FOS. - Rat Genome Database

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MiR-101a-3p Attenuated Pilocarpine-Induced Epilepsy by Downregulating c-FOS.

Authors: Geng, Jiefeng  Zhao, Haibiao  Liu, Xing  Geng, Junjie  Gao, Yuyuan  He, Bingzheng 
Citation: Geng J, etal., Neurochem Res. 2021 May;46(5):1119-1128. doi: 10.1007/s11064-021-03245-w. Epub 2021 Feb 9.
RGD ID: 405650612
Pubmed: PMID:33559830   (View Abstract at PubMed)
DOI: DOI:10.1007/s11064-021-03245-w   (Journal Full-text)

This study aimed to explore the effects and function of microRNA-101a-3p (miR-101a-3p) in epilepsy. Rat model of pilocarpine-induced epilepsy was established and the seizure frequency was recorded. Expression of miR-101a-3p and c-Fos in hippocampus tissues of Rat models were detected by qRT-PCR and western blot. Besides, we established a hippocampal neuronal culture model of acquired epilepsy using Mg2+ free medium to evaluate the effects of miR-101a-3p and c-Fos in vitro. Cells were transfected with miR-101a-3p mimic, si-c-FOS, miR-101a-3p + c-FOS and its corresponding controls. MTT assay was used to detect cell viability upon transfection. Flow cytometry was performed to determine the apoptosis rate. Western blot was performed to measure the protein expression of apoptosis-related proteins (Bcl-2, Bax, and cleaved caspase 3), autophagy-related proteins (LC3 and Beclin1) and c-FOS. The targeting relationship between miR-101a-3p and c-FOS was predicted and verified by TargetScan software and dual-luciferase reporter assay. The role of miR-101a-3p was validated using epilepsy rat models in vivo. Another Rat models of pilocarpine-induced epilepsy with miR-NC or miR-101a-3p injection were established to evaluate the effect of miR-101a-3p overexpression on epilepsy in vivo. MiR-101a-3p was downregulated while c-FOS was increased in hippocampus tissues of Rat model of pilocarpine-induced epilepsy. Overexpression of miR-101a-3p or c-FOS depletion promoted cell viability, inhibited cell apoptosis and autophagy. C-FOS was a target of miR-101a-3p and miR-101a-3p negatively regulated c-FOS expression to function in epilepsy. Overexpression of miR-101a-3p attenuated pilocarpine-induced epilepsy in Rats in vivo. This study indicated that miR-101a-3p could attenuate pilocarpine-induced epilepsy by repressing c-Fos expression.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FOSHumantemporal lobe epilepsy treatmentISOFos (Rattus norvegicus) RGD 
FosRattemporal lobe epilepsy treatmentIEP  RGD 
FosMousetemporal lobe epilepsy treatmentISOFos (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fos  (Fos proto-oncogene, AP-1 transcription factor subunit)

Genes (Mus musculus)
Fos  (FBJ osteosarcoma oncogene)

Genes (Homo sapiens)
FOS  (Fos proto-oncogene, AP-1 transcription factor subunit)


Additional Information