RGD Reference Report - ERK1/2 protein and mRNA levels in human blood are linked to smoking behavior. - Rat Genome Database

RGD Reference Report - ERK1/2 protein and mRNA levels in human blood are linked to smoking behavior. - Rat Genome Database

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ERK1/2 protein and mRNA levels in human blood are linked to smoking behavior.
Authors:	Lenz, Bernd Klafki, Hans-Wolfgang Hillemacher, Thomas Frieling, Helge Clepce, Marion Gossler, Andrea Thuerauf, Norbert Winterer, Georg Kornhuber, Johannes Bleich, Stefan
Citation:	Lenz B, etal., Addict Biol. 2012 Nov;17(6):1026-35. doi: 10.1111/j.1369-1600.2010.00264.x. Epub 2010 Nov 11.
RGD ID:	401966883
Pubmed:	PMID:21070506 (View Abstract at PubMed)
DOI:	DOI:10.1111/j.1369-1600.2010.00264.x (Journal Full-text)
From studies in cultured cells and animal models, nicotine and alcohol are known to regulate extracellular signal-regulated kinase 1 and 2 (ERK1/2). Alterations of ERK1/2 are thought to contribute to the drugs' rewarding effects. Accumulating evidence supports the importance of ERK1/2 in the molecular pathophysiology of depression and affective regulation in the hippocampus. We recently showed that the expression and phosphorylation of cyclic adenosine monophosphate response element (CRE)-binding protein (CREB) in human buffy coat were associated with smoking behavior. Because ERK1/2 is known to effect phosphorylation of CREB, the aim of the present study was to further elucidate whether cigarette smoking leads to alterations in terms of ERK1/2 in human buffy coat as well. In a comparison of 53 smokers with 146 non-smoking controls, we found significantly higher levels of ERK1/2 protein (P=0.004). In contrast, phospho-ERK1/2, phospho-/total-ERK1/2 ratio, mRNA-ERK1 and mRNA-ERK2 were not significantly different. Multiple regression analysis revealed a significant relation among the number of cigarettes smoked daily (R(2)=0.266, P=0.003), the Fagerström Test for Nicotine Dependence score (R(2)=0.149, P=0.032) and the mRNA expression of ERK1. Moreover, our analysis suggests that the mRNA expression of ERK2 might be linked to mood (model summary: R(2)=0.087, P=0.019; mRNA-ERK2: P=0.026). Given that the ERK1/2 signaling pathway plays an important role in the physiology and pathophysiology of affective and addictive behavior, our findings provide a rationale basis for additional mechanistic studies that may lead to the development of novel signaling pathway selective therapeutics in humans.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
MAPK1	Human	depressive disorder		IEP			RGD
Mapk1	Rat	depressive disorder		ISO	MAPK1 (Homo sapiens)		RGD
Mapk1	Mouse	depressive disorder		ISO	MAPK1 (Homo sapiens)		RGD

Genes (Rattus norvegicus)


Mapk1 (mitogen activated protein kinase 1)

Genes (Mus musculus)


Mapk1 (mitogen-activated protein kinase 1)

Genes (Homo sapiens)


MAPK1 (mitogen-activated protein kinase 1)