RGD Reference Report - Determinants of platelet conjugate formation with polymorphonuclear leukocytes or monocytes in whole blood. - Rat Genome Database

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Determinants of platelet conjugate formation with polymorphonuclear leukocytes or monocytes in whole blood.

Authors: Izzi, Benedetta  Pampuch, Agnieszka  Costanzo, Simona  Vohnout, Branislav  Iacoviello, Licia  Cerletti, Chiara  de Gaetano, Giovanni 
Citation: Izzi B, etal., Thromb Haemost. 2007 Dec;98(6):1276-84.
RGD ID: 329901666
Pubmed: PMID:18064325   (View Abstract at PubMed)

Following preliminary in-vitro experiments, platelet-leukocyte conjugates and their determinants were evaluated in citrated whole blood from 349 subjects (209 women, age 16-92 years) randomly recruited from the general population. Platelet activation by ADP/collagen but not leukocyte stimulation by fMLP or LTB4 resulted in formation of platelet conjugates with PMN or monocytes. In the population study, mixed cell conjugates, platelet P-selectin and leukocyte CD11b were measured by flow cytometry both at baseline and after in-vitro stimulation with ADP/collagen. The latter significantly increased platelet conjugates with either PMN or monocytes, platelet P-selectin and leukocyte CD11b expression. Platelet count significantly correlated with platelet-PMN, platelet-monocyte conjugates and P-selectin both at baseline and upon stimulation. In all conditions, both conjugate levels correlated with each other, when adjusted for gender, age and platelet count. Age correlated with platelet-PMN conjugate numbers in basal and stimulated conditions and with basal P-selectin. ADP/collagen stimulation resulted in higher P-selectin and conjugates values in women. Among risk factors, a significant correlation was found between conjugate and glucose levels. In conclusion, the presence and formation in whole blood from a large population of platelet-leukocyte conjugates reflects primary platelet - but not leukocyte - activation and varies with gender, age, platelet count and blood glucose.



Gene-Chemical Interaction Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ITGAMHumanleukotriene B4 increases expression EXP LTB4 increases expression of ITGAM protein on neutrophils and monocytesRGD 
ItgamRatleukotriene B4 increases expression ISOITGAM (Homo sapiens)LTB4 increases expression of ITGAM protein on neutrophils and monocytesRGD 
ItgamMouseleukotriene B4 increases expression ISOITGAM (Homo sapiens)LTB4 increases expression of ITGAM protein on neutrophils and monocytesRGD 
ITGAMHumanN-formyl-L-methionyl-L-leucyl-L-phenylalanine increases expression EXP fMLP increases expression of ITGAM protein on neutrophils and monocytesRGD 
ItgamRatN-formyl-L-methionyl-L-leucyl-L-phenylalanine increases expression ISOITGAM (Homo sapiens)fMLP increases expression of ITGAM protein on neutrophils and monocytesRGD 
ItgamMouseN-formyl-L-methionyl-L-leucyl-L-phenylalanine increases expression ISOITGAM (Homo sapiens)fMLP increases expression of ITGAM protein on neutrophils and monocytesRGD 

Objects Annotated

Genes (Rattus norvegicus)
Itgam  (integrin subunit alpha M)

Genes (Mus musculus)
Itgam  (integrin alpha M)

Genes (Homo sapiens)
ITGAM  (integrin subunit alpha M)


Additional Information