RGD Reference Report - Greater stress protein expression enhanced by combined prostaglandin A1 and lithium in a rat model of focal ischemia. - Rat Genome Database

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Greater stress protein expression enhanced by combined prostaglandin A1 and lithium in a rat model of focal ischemia.

Authors: Xu, XH  Hua, YN  Zhang, HL  Wu, JC  Miao, YZ  Han, R  Gu, ZL  Qin, ZH 
Citation: Xu XH, etal., Acta Pharmacol Sin. 2007 Aug;28(8):1097-104.
RGD ID: 2325742
Pubmed: PMID:17640469   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1745-7254.2007.00624.x   (Journal Full-text)

AIM: To investigate the effects of lithium (Li) and prostaglandin A1 (PGA1) on the expression of heat shock factor 1 (HSF-1), heat shock proteins (HSP), and apoptosis protease activating factor-1 (Apaf-1) induced by permanent focal ischemia in rats. METHODS: The rats were pretreated with a subcutaneous (sc) injection of Li for 2 d or a single intracerebral ventricle (icv) administration of PGA1 for 15 min before ischemic insult, or a combination of Li (sc, 1 mEq/kg, 2 d) and PGA1 (icv, 15 min prior to ischemic insult). Brain ischemia was induced by the permanent middle cerebral artery occlusion (pMCAO). Twenty-four hours after the occlusion, the expression of HSF-1, HSP, and Apaf-1 in the ischemic striatum were examined with Western blot analysis. RESULTS: The expression of HSF-1, heme oxygenase-1 (HO-1), HSP90alpha, and Apaf-1 were significantly increased, but the expression of HSP90beta was significantly decreased 24 h after the pMCAO. PGA1 and Li and their combination significantly enhanced the ischemia-induced elevation in the levels of HSF-1, HO-1, and HSP90alpha, and recovered HSP90beta expression, but decreased Apaf-1 levels in the ischemic striatum. CONCLUSION: The present study demonstrates that PGA1 and Li have synergistic effects on the enhancement of the expression of HSP, suggesting that the synergistic effects of PGA1 and Li in the rat model of permanent focal cerebral ischemia may be mediated by the enhancement expression of HSP expression and the downregulation of Apaf-1. Our studies suggest that combined PGA1 and Li may have potential clinical value for the treatment of stroke.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
APAF1Humanbrain ischemia  ISOApaf1 (Rattus norvegicus)protein:increased expression:striatumRGD 
Apaf1Ratbrain ischemia  IEP protein:increased expression:striatumRGD 
Apaf1Mousebrain ischemia  ISOApaf1 (Rattus norvegicus)protein:increased expression:striatumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Apaf1  (apoptotic peptidase activating factor 1)

Genes (Mus musculus)
Apaf1  (apoptotic peptidase activating factor 1)

Genes (Homo sapiens)
APAF1  (apoptotic peptidase activating factor 1)


Additional Information