RGD Reference Report - Redistribution of myosin VI from top to base of proximal tubule microvilli during acute hypertension. - Rat Genome Database

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Redistribution of myosin VI from top to base of proximal tubule microvilli during acute hypertension.

Authors: Yang, LE  Maunsbach, AB  Leong, PK  McDonough, AA 
Citation: Yang LE, etal., J Am Soc Nephrol. 2005 Oct;16(10):2890-6. Epub 2005 Aug 17.
RGD ID: 2314963
Pubmed: PMID:16107581   (View Abstract at PubMed)
DOI: DOI:10.1681/ASN.2005040366   (Journal Full-text)

During acute hypertension, Na(+)/H(+) exchangers (NHE3) retract from top to base of proximal tubule microvilli (MV) and Na(+) reabsorption decreases in proximal tubule. This study aimed to determine whether the actin-based motor myosin VI coordinately retracts with NHE3 in response to acute hypertension. BP was raised approximately 50 mmHg in rats for 20 to 30 min or sham treated, and kidneys were analyzed by subcellular fractionation or microscopy. During acute hypertension, myosin VI redistributed from low density apical MV-enriched membranes (from 23 +/- 2.4 to 11.4 +/- 2.2%) into higher density membranes (from 23.2 +/- 0.7 to 36.9 +/- 2.6%). By confocal microscopy, myosin VI was detected over the whole length of the MV in controls, then became completely focused at the base of MV during acute hypertension. For electron microscopic analysis using immunogold labeling, MV were divided into five zones from top (z1) to base (z5). In controls, myosin VI was evenly distributed through the five MV zones. In acute hypertension, myosin VI decreased in z1 (from 20.6 +/- 1.9 to 10.5 +/- 2.3%) and z2 (from 21.0 +/- 2.0 to 13.2 +/- 1.4%) and increased in z5 (from 21.1 +/- 3.3 to 38.6 +/- 3.0%). These results provide the first observation that acute hypertension causes myosin VI redistribution and support the idea that myosin VI may serve as the molecular motor for NHE3 retraction from top to base of MV during acute hypertension.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MYO6Humanhypertension  ISOMyo6 (Rattus norvegicus)protein:altered localization:renal proximal tubule and microvillusRGD 
Myo6Mousehypertension  ISOMyo6 (Rattus norvegicus)protein:altered localization:renal proximal tubule and microvillusRGD 
Myo6Rathypertension  IDA protein:altered localization:renal proximal tubule and microvillusRGD 

Objects Annotated

Genes (Rattus norvegicus)
Myo6  (myosin VI)

Genes (Mus musculus)
Myo6  (myosin VI)

Genes (Homo sapiens)
MYO6  (myosin VI)


Additional Information