RGD Reference Report - Investigating structural and biochemical correlates of ganglion cell dysfunction in streptozotocin-induced diabetic rats. - Rat Genome Database

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Investigating structural and biochemical correlates of ganglion cell dysfunction in streptozotocin-induced diabetic rats.

Authors: Bui, BV  Loeliger, M  Thomas, M  Vingrys, AJ  Rees, SM  Nguyen, CT  He, Z  Tolcos, M 
Citation: Bui BV, etal., Exp Eye Res. 2009 Jun;88(6):1076-83. Epub 2009 Feb 7.
RGD ID: 2314507
Pubmed: PMID:19450451   (View Abstract at PubMed)
DOI: DOI:10.1016/j.exer.2009.01.009   (Journal Full-text)

The aim of this study was to determine whether inner retinal dysfunction in diabetic rats is correlated with structural and/or biochemical changes in the retina and optic nerve. Using the electroretinogram (ERG; -5.83 to 1.28 log cd.s.m(-2)) retinal function (photoreceptor, bipolar, amacrine and ganglion cell components) was measured in control (n=13; citrate buffer) and diabetic (n=13; streptozotocin, STZ, 50 mg kg(-1)) rats, 12 weeks following treatment. Retinae and optic nerves were analyzed for structural changes and retinae were assessed for alterations in growth factor/cytokine expression using quantitative real-time PCR. We found that phototransduction efficiency was reduced 12 weeks after STZ-induced diabetes (-30%), leading to reduced amplitude of ON-bipolar (-18%) and amacrine cell (-29%) dominated responses; ganglion cell dysfunction (-84%) was more profound. In the optic nerve, nerve fascicle area and myelin sheath thickness were reduced (p<0.05), whereas the ratio of blood vessels and connective tissue to total nerve cross-sectional area was increased (p<0.05) in diabetic compared to control rats. In the retina, connective tissue growth factor (CTGF), transforming growth factor beta, type 2 receptor (TGFbeta-r2) mRNA and platelet-derived growth factor B (PDGF-B) mRNA were increased (p<0.035). Reduced ganglion cell function was correlated with increased CTGF and TGFbeta-r2, but not PDGF-B mRNA. In summary, the ganglion cell component exhibited the greatest level of dysfunction within the ERG components examined after 12 weeks of STZ-induced diabetes; the level correlated with increased CTGF and TGFbeta-r2 mRNA, but not with gross morphological changes in the retina or optic nerve.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CCN2Humandiabetic retinopathy  ISOCcn2 (Rattus norvegicus)associated with Diabetes Mellitus more ...RGD 
Ccn2Ratdiabetic retinopathy  IEP associated with Diabetes Mellitus more ...RGD 
Ccn2Mousediabetic retinopathy  ISOCcn2 (Rattus norvegicus)associated with Diabetes Mellitus more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccn2  (cellular communication network factor 2)

Genes (Mus musculus)
Ccn2  (cellular communication network factor 2)

Genes (Homo sapiens)
CCN2  (cellular communication network factor 2)


Additional Information