RGD Reference Report - In vivo delivery of a XIAP (BIR3-RING) fusion protein containing the protein transduction domain protects against neuronal death induced by seizures. - Rat Genome Database

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In vivo delivery of a XIAP (BIR3-RING) fusion protein containing the protein transduction domain protects against neuronal death induced by seizures.

Authors: Li, T  Fan, Y  Luo, Y  Xiao, B  Lu, C 
Citation: Li T, etal., Exp Neurol. 2006 Feb;197(2):301-8. Epub 2005 Dec 5.
RGD ID: 2314393
Pubmed: PMID:16336964   (View Abstract at PubMed)
DOI: DOI:10.1016/j.expneurol.2005.08.021   (Journal Full-text)

The prevention of cell apoptosis is a promising strategy for neuroprotection against brain injury in seizures. X-linked inhibitor of apoptosis protein (XIAP) is regarded as the most potent inhibitor of cell apoptosis. In the present study, we fused the protein transduction domain (PTD) of Antennapedia Homeodomain of Drosophila (AntpHD) to XIAP (BIR3-RING) and explored the neuroprotective effect of XIAP in rats with seizures induced by kainic acid (KA). KA triggered neuronal death in the ipsilateral CA3 subfield of the hippocampus and activation of caspase-3 and -9. PTD-XIAP fusion protein can be delivered into cos7 cells in vitro. We used intraperitoneal injection to deliver the PTD-XIAP fusion protein which can enter into brain, significantly decrease the TUNEL positive cells and increase the number of surviving cells in the ipsilateral CA3 subfield of the hippocampus at 24 h after KA-induced seizures. Furthermore, PTD-XIAP fusion protein attenuated activated caspase-3 and -9. These results demonstrate the neuroprotective effect of PTD-XIAP fusion protein against brain injury possibly through the inhibition of caspase. The significance of these findings in the treatment of epilepsy still needs to be extensively studied.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
XiapRatnegative regulation of neuron apoptotic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Casp3Ratcysteine-type peptidase activity  IDA  RGD 
Casp9Ratcysteine-type peptidase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Casp3  (caspase 3)
Casp9  (caspase 9)
Xiap  (X-linked inhibitor of apoptosis)


Additional Information