RGD Reference Report - Barttin modulates trafficking and function of ClC-K channels. - Rat Genome Database

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Barttin modulates trafficking and function of ClC-K channels.

Authors: Scholl, U  Hebeisen, S  Janssen, AG  Muller-Newen, G  Alekov, A  Fahlke, C 
Citation: Scholl U, etal., Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11411-6. Epub 2006 Jul 18.
RGD ID: 2313579
Pubmed: PMID:16849430   (View Abstract at PubMed)
PMCID: PMC1544099   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0601631103   (Journal Full-text)

Barttin is an accessory subunit of a subgroup of ClC-type chloride channels expressed in renal and inner ear epithelia. In this study, we examined the effects of barttin on two ClC-K channel isoforms, rat ClC-K1 and human ClC-Kb, using heterologous expression, patch clamping, confocal imaging, and flow cytometry. In the absence of barttin, only a small percentage of rClC-K1 and hClC-Kb channels are inserted into the plasma membrane. Coexpression of barttin enhances surface membrane insertion and furthermore modifies permeation and gating of ClC-K channels. hClC-Kb channels are nonfunctional without barttin and require the coexpressed accessory subunit to become anion conducting. In contrast, rClC-K1 channels are active without barttin, but at the cost of reduced unitary conductance as well as altered voltage dependence of activation. We mapped the separate functions of barttin to structural domains by a deletion analysis. Whereas the transmembrane core is necessary and sufficient to promote ClC-K channel exit from the endoplasmic reticulum, a short cytoplasmic segment following the second transmembrane helix modifies the unitary conductance. The entire cytoplasmic carboxyl terminus affects the open probability of ClC-K channels. The multiple functions of barttin might be necessary for a tight adjustment of epithelial Cl(-) conductances to ensure a precise regulation of body salt content and endocochlear potential.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
voltage-gated monoatomic ion channel activity  IDA 2313579 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Clcnka  (chloride voltage-gated channel Ka)


Additional Information