RGD Reference Report - Effect of thyroid hormones on peptidylglycine alpha-amidating monooxygenase gene expression in anterior pituitary gland: transcriptional studies and messenger ribonucleic acid stability. - Rat Genome Database

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Effect of thyroid hormones on peptidylglycine alpha-amidating monooxygenase gene expression in anterior pituitary gland: transcriptional studies and messenger ribonucleic acid stability.

Authors: Fraboulet, S  Boudouresque, F  Delfino, C  Fina, F  Oliver, C  Ouafik, L 
Citation: Fraboulet S, etal., Endocrinology. 1996 Dec;137(12):5493-501.
RGD ID: 2302427
Pubmed: PMID:8940376   (View Abstract at PubMed)
DOI: DOI:10.1210/endo.137.12.8940376   (Journal Full-text)

Peptidylglycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is a multifunctional protein containing two enzymes that act sequentially to catalyze the alpha-amidation of neuroendocrine peptides. The regulatory mechanism(s) involved in the tissue-specific induction of PAM messenger RNA (mRNA) by thyroid status have been investigated in rat anterior pituitary gland. In this tissue, cellular PAM mRNA increases in response to hypothyroidism (4- to 7-fold above basal levels). To gain further insight into this pretranslational control, nuclear in vitro run-on transcription assays were performed. Using PAM complementary DNAs and intronic probe, we showed that the transcriptional rate of rat pituitary PAM gene in isolated nuclei was not altered by thyroid status. Primary rat pituitary cells cultures from hypo- and euthyroid rats in the presence of actinomycin D showed that hypothyroidism increased the half-life of PAM mRNA from 9-10 h to 15-17 h. Taken together, these data suggest that hypothyroidism induces PAM mRNA levels by increasing its stability in the cytoplasm.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PAMHumanhypothyroidism  ISOPam (Rattus norvegicus)mRNA:increased stability:pituitary glandRGD 
PamRathypothyroidism  IEP mRNA:increased stability:pituitary glandRGD 
PamMousehypothyroidism  ISOPam (Rattus norvegicus)mRNA:increased stability:pituitary glandRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pam  (peptidylglycine alpha-amidating monooxygenase)

Genes (Mus musculus)
Pam  (peptidylglycine alpha-amidating monooxygenase)

Genes (Homo sapiens)
PAM  (peptidylglycine alpha-amidating monooxygenase)


Additional Information