RGD Reference Report - CpG island methylation in Schistosoma- and non-Schistosoma-associated bladder cancer. - Rat Genome Database

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CpG island methylation in Schistosoma- and non-Schistosoma-associated bladder cancer.

Authors: Gutierrez, MI  Siraj, AK  Khaled, H  Koon, N  El-Rifai, W  Bhatia, K 
Citation: Gutierrez MI, etal., Mod Pathol. 2004 Oct;17(10):1268-74.
RGD ID: 2301233
Pubmed: PMID:15154012   (View Abstract at PubMed)
DOI: DOI:10.1038/modpathol.3800177   (Journal Full-text)

Urothelial carcinomas (TCC) constitute the vast majority of bladder cancers in most of the world. On the other hand, squamous cell bladder carcinoma, a rare subtype in the Western world, is a common subtype in areas with endemic Schistosoma infection. Although schistosomal infection has been reported to influence DNA methylation, the pattern and extent of CpG island hypermethylation in squamous cell carcinomas remain unknown. In this study, we used methylation-specific PCR to characterize 12 cancer-related genes in 41 bladder cancer samples from Egypt (31 squamous cell carcinomas (SCC), 21 of them associated with Schistosoma and 10 TCC, five of which were Schistosoma-associated). The genes analyzed included E-cadherin, DAP-Kinase, O6MGMT, p14, p15, p16, FHIT, APC, RASSF1A, GSTP1, RARbeta and p73. Methylation of at least one gene was detected in all squamous cell tumors except two, and 45% of samples had at least three methylated genes. The average methylation index was 0.24, corresponding to three of the 12 analyzed genes. Schistosoma-associated tumors had more genes methylated than non-Schistosoma tumors (average MI: 0.29 vs 0.14) (P = 0.027). Although the extent of methylation in TCC (average MI: 0.16) was lower than in squamous cell carcinomas (SCC), the overall profile of methylation was similar, with Schistosoma-associated cases having a higher methylation index. Our results suggest that schistosomal involvement associates with a greater degree of epigenetic changes in the bladder epithelium.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FHITHumanurinary bladder cancer  IDA associated with Schistosomiasis and DNA:hypermethylation:urinary bladderRGD 
FhitRaturinary bladder cancer  ISOFHIT (Homo sapiens)associated with Schistosomiasis and DNA:hypermethylation:urinary bladderRGD 
FhitMouseurinary bladder cancer  ISOFHIT (Homo sapiens)associated with Schistosomiasis and DNA:hypermethylation:urinary bladderRGD 

Objects Annotated

Genes (Rattus norvegicus)
Fhit  (fragile histidine triad diadenosine triphosphatase)

Genes (Mus musculus)
Fhit  (fragile histidine triad gene)

Genes (Homo sapiens)
FHIT  (fragile histidine triad diadenosine triphosphatase)


Additional Information