RGD Reference Report - Increased expression of schwannoma-derived growth factor (SDGF) mRNA in rat tumor cells: involvement of SDGF in the growth promotion of rat gliomas. - Rat Genome Database

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Increased expression of schwannoma-derived growth factor (SDGF) mRNA in rat tumor cells: involvement of SDGF in the growth promotion of rat gliomas.

Authors: Mishima, K  Asai, A  Sugiyama, A  Miyagi, Y  Kitanaka, C  Kagaya, S  Kirino, T  Kuchino, Y 
Citation: Mishima K, etal., Int J Cancer. 1996 May 3;66(3):352-7.
RGD ID: 2292668
Pubmed: PMID:8621257   (View Abstract at PubMed)
DOI: DOI:10.1002/(SICI)1097-0215(19960503)66:3<352::AID-IJC15>3.0.CO;2-D   (Journal Full-text)

Schwannoma-derived growth factor (SDGF) is a member of the epidermal growth factor (EGF) family, having mitogenic activity on rat astrocytes, fibroblasts and Schwann cells. The SDGF gene is significantly expressed in the newborn rat lung and in the adult rat sciatic nerve. However, except for one rat schwannoma cell line, from which SDGF and its cDNA were isolated, nothing is known about SDGF expression in established tumor cell lines. We examined the expression level of the SDGF gene in a variety of rat tumor cell lines by Northern blotting and found that it was increased in 11 of 25 established lines. The most abundant SDGF mRNA, which was about 50-fold higher than in the newborn rat lung, was expressed in rat liver adenoma dRLa74 cells. In rat glioma cell lines, such as C6, 9L and T9, and in the rat hepatoma dRLh84 and H411E cells, the SDGF expression level was about 10-fold higher than in the newborn rat lung. In 8 of 13 cell lines expressing SDGF mRNA, the EGF receptor (EGFR) gene, the product of which is regarded as a functional receptor of SDGF, was co-expressed. In addition, transfected gene-dependent anti-sense SDGF RNA expression under the control of the human metallothionein promoter significantly suppressed the in vitro growth as well as in vivo tumorigenicity of 9L glioma cells. Our results suggest that SDGF acts as an autocrine growth factor in the development and growth of rat tumors such as gliomas.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
high grade glioma  ISOAreg (Rattus norvegicus)2292668; 2292668 RGD 
high grade glioma  IMP 2292668 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cell population proliferation  IMP 2292668 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Areg  (amphiregulin)

Genes (Mus musculus)
Areg  (amphiregulin)

Genes (Homo sapiens)
AREG  (amphiregulin)


Additional Information