RGD Reference Report - Distinct and overlapping patterns of localization of bone morphogenetic protein (BMP) family members and a BMP type II receptor during fracture healing in rats. - Rat Genome Database

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Distinct and overlapping patterns of localization of bone morphogenetic protein (BMP) family members and a BMP type II receptor during fracture healing in rats.

Authors: Onishi, T  Ishidou, Y  Nagamine, T  Yone, K  Imamura, T  Kato, M  Sampath, TK  Ten Dijke, P  Sakou, T 
Citation: Onishi T, etal., Bone. 1998 Jun;22(6):605-12.
RGD ID: 2289041
Pubmed: PMID:9626398   (View Abstract at PubMed)

Bone morphogenetic proteins (BMPs) and their receptors (BMPRs) are thought to play an important role in bone morphogenesis. The purpose of this study was to determine the locations of BMP-2/-4, osteogenic protein-1 (OP-1, also termed BMP-7), and BMP type II receptor (BMPR-II) during rat fracture healing by immunostaining, and thereby elucidate the possible roles of the BMPs and BMPR-II in intramembranous ossification and endochondral ossification. In the early stage of fracture repair, the expression of BMP-2/-4 and OP-1 was strongly induced in the thickened periosteum near the fracture ends, and coincided with an enhanced expression of BMPR-II. On day 7 after fracture, staining for BMP-2/-4 and OP-1 immunostaining was increased in various types of chondrocytes, and was strong in fibroblast-like spindle cells and proliferating chondrocytes in endochondral bone. On day 14 after fracture, staining with OP-1 antibody disappeared in proliferating and mature chondrocytes, while BMP-2/-4 staining continued in various types of chondrocytes until the late stage. In the newly formed trabecular bone, BMP-2/-4 and OP-1 were present at various levels. BMPR-II was actively expressed in both intramembranous ossification and endochondral ossification. Additionally, immunostaining for BMP-2/-4 and OP-1 was observed in multinucleated osteoclast-like cells on the newly formed trabecular bone, along with BMPR-II. In reference to our previous study of BMP type I receptors (BMPR-IA and BMPR-IB), BMPR-II was found to be co-localized with BMPR-IA and BMPR-IB. BMP-2/-4 and OP-1 antibodies exhibited distinct and overlapping immunostaining patterns during fracture repair. OP-1 may act predominantly in the initial phase of endochondral ossification, while BMP-2/-4 acts throughout this process. Thus, these findings suggested that BMPs acting through their BMP receptors may play major roles in modulating the sequential events leading to bone formation.



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Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Bmp7Ratcollagen-containing extracellular matrix  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Bmp7  (bone morphogenetic protein 7)
Bmpr2  (bone morphogenetic protein receptor type 2)

Genes (Mus musculus)
Bmp7  (bone morphogenetic protein 7)
Bmpr2  (bone morphogenetic protein receptor type 2)

Genes (Homo sapiens)
BMP7  (bone morphogenetic protein 7)
BMPR2  (bone morphogenetic protein receptor type 2)


Additional Information