RGD Reference Report - Caspase-cleaved cytokeratin 18 and 20 S proteasome in liver degeneration. - Rat Genome Database

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Caspase-cleaved cytokeratin 18 and 20 S proteasome in liver degeneration.

Authors: Hetz, Hubert  Hoetzenecker, Konrad  Hacker, Stefan  Faybik, Peter  Pollreisz, Andreas  Moser, Bernhard  Roth, Georg  Hoetzenecker, Wolfram  Lichtenauer, Michael  Klinger, Markus  Krenn, Claus Georg  Ankersmit, Hendrik Jan 
Citation: Hetz H, etal., J Clin Lab Anal. 2007;21(5):277-81. doi: 10.1002/jcla.20180.
RGD ID: 18337491
Pubmed: PMID:17847110   (View Abstract at PubMed)
PMCID: PMC6648973   (View Article at PubMed Central)
DOI: DOI:10.1002/jcla.20180   (Journal Full-text)

Apoptosis of epithelial hepatocytes plays a pivotal role in acute as well as in chronic liver diseases. The cleavage of cytokeratin-18 (CK-18) by caspases is an early event in the apoptotic process. We therefore sought to investigate serum levels of CK-18 and 20S proteasome in patients with liver cirrhosis, primary graft dysfunction (PDF), and acute liver failure (ALF), and in healthy volunteers. Enzyme-linked immunosorbent assay (ELISA) was utilized to measure the concentration of M30, a fragment of CK-18 cleaved at Asp396 (M30 neoantigen), and the concentration of 20S proteasome. Serum levels of the CK-18 neoepitope M30 were significantly increased in ALF, primary graft dysfunction, and liver cirrhosis vs. healthy controls (1,993.6+/-124.7 U/L, 2,238.1+/-235.9 U/L, and 673.6+/-86.5 U/L vs. 66.8+/-29.1 U/L, respectively, P<0.001). Similar results were detected with the evaluation of 20S proteasome (124,014.5+/-13,235.6 ng/mL, 76,993.2+/-15,720.1 ng/mL, and 2,395.9+/-1,098.2 ng/mL vs. 1,074.5+/-259.4 ng/mL, respectively; P<0.001). Detection of CK-18 neoepitope M30 and 20S proteasome may represent a novel marker of tracing apoptotic epithelium, respectively mirroring degenerative liver processes in affected patient population.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
KRT18HumanAcute Liver Failure  IEP protein:increased degradation:serum (human)RGD 
Krt18RatAcute Liver Failure  ISOKRT18 (Homo sapiens)protein:increased degradation:serum (human)RGD 
Krt18MouseAcute Liver Failure  ISOKRT18 (Homo sapiens)protein:increased degradation:serum (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Krt18  (keratin 18)

Genes (Mus musculus)
Krt18  (keratin 18)

Genes (Homo sapiens)
KRT18  (keratin 18)


Additional Information