RGD Reference Report - Cross-talk between protein kinases Czeta and B in cyclic AMP-mediated sodium taurocholate co-transporting polypeptide translocation in hepatocytes. - Rat Genome Database

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Cross-talk between protein kinases Czeta and B in cyclic AMP-mediated sodium taurocholate co-transporting polypeptide translocation in hepatocytes.

Authors: McConkey, M  Gillin, H  Webster, CR  Anwer, MS 
Citation: McConkey M, etal., J Biol Chem. 2004 May 14;279(20):20882-8. Epub 2004 Mar 8.
RGD ID: 1642700
Pubmed: PMID:15007074   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M309988200   (Journal Full-text)

Cyclic AMP stimulates taurocholate (TC) uptake and sodium taurocholate co-transporting polypeptide (Ntcp) translocation in hepatocytes via the phosphoinositide-3 kinase (PI3K) signaling pathway. The aim of the present study was to determine whether protein kinase (PK) Czeta, one of the downstream mediators of the PI3K signaling pathway, is involved in cAMP-mediated stimulation of TC uptake. Studies were conducted in isolated rat hepatocytes and in HuH-7 cells stably transfected with rat liver Ntcp (HuH-Ntcp cells). Studies in hepatocytes showed that cAMP activates PKCzeta in a PI3K-dependent manner without inducing translocation of PKCzeta to the plasma membrane. Inhibition of cAMP-induced PKCzeta activity by myristoylated PKC (zeta/lambda) pseudosubstrate, a specific inhibitor of PKCzeta, and Go 6850, a PKC inhibitor, resulted in inhibition of cAMP-induced increases in TC uptake and Ntcp translocation. Studies in HuH-Ntcp cells showed that inhibition of cAMP-induced PKCzeta activation by dominant-negative (DN) PKCzeta resulted in inhibition of cAMP-induced increases in TC uptake and Ntcp translocation. DN PKCzeta also inhibited wild-type PKCzeta-induced increases in PKCzeta activity, TC uptake, and Ntcp translocation. Myristoylated PKC (zeta/lambda) pseudosubstrate and DN PKCzeta also inhibited cAMP-induced activation of PKB in hepatocytes and HuH-Ntcp cells, respectively. Neither DN PKB nor constitutively active PKB affected cAMP-induced activation of PKCzeta, and wild-type PKCzeta did not activate PKB. Taken together, these results suggest that cAMP-induced activation of PKB is dependent on cAMP-induced stimulation of PKCzeta. It is proposed that cAMP-induced Ntcp translocation involves the activation of the PI3K/PKCzeta signaling pathway followed by the activation of the PI3K/PKB signaling pathway.




Biological Process

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
PrkczRatactivation of protein kinase B activity  IMP in hepatocytesRGD 
Pdk1Ratcell surface receptor signaling pathway  IMP neuregulinNrg1-Erbb3 signaling pathwayRGD 
PrkczRatcell surface receptor signaling pathway  IMP neuregulinNrg1-Erbb3 signaling pathwayRGD 
PrkczRatpositive regulation of protein transport  IMP translocation of Ntcp to plasma membraneRGD 

RGD Manual Annotations


  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Erbb3Ratepidermal growth factor/neuregulin signaling pathway   IMP Neuregulin stimulation of glucose transport in muscle cellsRGD 
Pdk1Ratepidermal growth factor/neuregulin signaling pathway   IMP Neuregulin stimulation of glucose transport in muscle cellsRGD 
PrkczRatepidermal growth factor/neuregulin signaling pathway   IMP Neuregulin stimulation of glucose transport in muscle cellsRGD 

Genes (Rattus norvegicus)
Erbb3  (erb-b2 receptor tyrosine kinase 3) Pdk1  (pyruvate dehydrogenase kinase 1) Prkcz  (protein kinase C, zeta)