RGD Reference Report - Matrix metalloproteinases are down-regulated in rat lenses exposed to oxidative stress. - Rat Genome Database

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Matrix metalloproteinases are down-regulated in rat lenses exposed to oxidative stress.

Authors: John, M  Jaworski, C  Chen, Z  Subramanian, S  Ma, W  Sun, F  Li, D  Spector, A  Carper, D 
Citation: John M, etal., Exp Eye Res. 2004 Dec;79(6):839-46.
RGD ID: 1582600
Pubmed: PMID:15642321   (View Abstract at PubMed)
DOI: DOI:10.1016/j.exer.2004.08.025   (Journal Full-text)

Matrix metalloproteinases are important biological effectors of tissue remodelling. Increased MMP expression occurs during injury, inflammation, cellular transformation, and oxidative stress. Oxidative stress in the lens, a causal factor in cataractogenesis, has been shown to induce MMP secretion. The objective of this study was to assess the expression of MMPs and their regulators in an oxidative stress model of cataract, where epithelial cell death and cortical fibre cell swelling occurs in rat lenses after exposure to riboflavin, oxygen, and light. Two time points (4 and 7 hr of exposure) were chosen in order to compare transparent lenses with partially opaque lenses. MMP activity, protein, and mRNA levels were measured. The results show that MMP-2, MMP-9, MT1-MMP, and MT3-MMP are down-regulated by oxidative stress and that the down-regulation is most likely due to reduced gene transcription. In contrast, genes for catalase, glutathione peroxidase, and GAPDH are essentially unaffected, while beta-actin mRNA and protein levels are markedly increased at both time points. The down-regulation of MMPs occurs in lenses still seemingly transparent after 4 hr of exposure, indicating that reduced MMP activity is a relatively early response to the oxidative stress. Moreover, in our model system, MMP inhibition, not induction, is associated with cataractogenesis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mmp14Ratresponse to oxidative stress  IEP  RGD 
Mmp2Ratresponse to oxidative stress  IDA  RGD 
Mmp9Ratresponse to oxidative stress  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mmp14  (matrix metallopeptidase 14)
Mmp2  (matrix metallopeptidase 2)
Mmp9  (matrix metallopeptidase 9)


Additional Information