RGD Reference Report - Neuronal expression of the NADPH oxidase NOX4, and its regulation in mouse experimental brain ischemia. - Rat Genome Database

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Neuronal expression of the NADPH oxidase NOX4, and its regulation in mouse experimental brain ischemia.

Authors: Vallet, P  Charnay, Y  Steger, K  Ogier-Denis, E  Kovari, E  Herrmann, F  Michel, JP  Szanto, I 
Citation: Vallet P, etal., Neuroscience. 2005;132(2):233-8.
RGD ID: 1580980
Pubmed: PMID:15802177   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuroscience.2004.12.038   (Journal Full-text)

Ischemia-induced neuronal damage has been linked to elevated production of reactive oxygen species (ROS) both in animal models and in humans. NADPH oxidase enzymes (NOX-es) are a major enzymatic source of ROS, but their role in brain ischemia has not yet been investigated. The present study was carried out to examine the expression of NOX4, one of the new NADPH oxidase isoforms in a mouse model of focal permanent brain ischemia. We demonstrate that NOX4 is expressed in neurons using in situ hybridization and immunohistochemistry. Ischemia, induced by middle cerebral artery occlusion resulted in a dramatic increase in cortical NOX4 expression. Elevated NOX4 mRNA levels were detectable as early as 24 h after the onset of ischemia and persisted throughout the 30 days of follow-up period, reaching a maximum between days 7 and 15. The early onset suggests neuronal reaction, while the peak period corresponds to the time of neoangiogenesis occurring mainly in the peri-infarct region. The occurrence of NOX4 in the new capillaries was confirmed by immunohistochemical staining. In summary, our paper reports the presence of the ROS producing NADPH oxidase NOX4 in neurons and demonstrates an upregulation of its expression under ischemic conditions. Moreover, a role for NOX4 in ischemia/hypoxia-induced angiogenesis is suggested by its prominent expression in newly formed capillaries.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NOX4Humanbrain ischemia  ISONox4 (Mus musculus)mRNA and protein:increased expression:cerebral cortex (human)RGD 
Nox4Ratbrain ischemia  ISONox4 (Mus musculus)mRNA and protein:increased expression:cerebral cortex (human)RGD 
Nox4Mousebrain ischemia  IEP mRNA and protein:increased expression:cerebral cortex (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nox4  (NADPH oxidase 4)

Genes (Mus musculus)
Nox4  (NADPH oxidase 4)

Genes (Homo sapiens)
NOX4  (NADPH oxidase 4)


Additional Information