RGD Reference Report - Expression and localization of Na-driven Cl-HCO(3)(-) exchanger (SLC4A8) in rodent CNS. - Rat Genome Database

RGD Reference Report - Expression and localization of Na-driven Cl-HCO(3)(-) exchanger (SLC4A8) in rodent CNS. - Rat Genome Database

Submit Data | Help | Video Tutorials | News | Publications | Download | REST API | Citing RGD | Contact

General

Expression and localization of Na-driven Cl-HCO(3)(-) exchanger (SLC4A8) in rodent CNS.
Authors:	Chen, L-M Kelly, M L Parker, M D Bouyer, P Gill, H S Felie, J M Davis, B A Boron, W F
Citation:	Chen LM, etal., Neuroscience. 2008 Apr 22;153(1):162-74. doi: 10.1016/j.neuroscience.2008.02.018. Epub 2008 Feb 21.
RGD ID:	155230773
Pubmed:	PMID:18359573 (View Abstract at PubMed)
PMCID:	PMC2905791 (View Article at PubMed Central)
DOI:	DOI:10.1016/j.neuroscience.2008.02.018 (Journal Full-text)
The Na(+)-driven Cl-HCO(3) exchanger (NDCBE or SLC4A8) is a member of the solute carrier 4 (SLC4) family of HCO(3)(-) transporters, which includes products of 10 genes with similar sequences. Most SLC4 members play important roles in regulating intracellular pH (pH(i)). Physiological studies suggest that NDCBE is a major pH(i) regulator in at least hippocampal (HC) pyramidal neurons. We generated a polyclonal rabbit antibody directed against the first 18 residues of the cytoplasmic N terminus (Nt) of human NDCBE. By Western blotting, the antibody distinguishes NDCBE-as a purified Nt peptide or a full-length transporter (expressed in Xenopus oocytes)-from other Na(+)-coupled HCO(3)(-) transporters. By Western blotting, the antiserum recognizes an approximately 135-kDa band in several brain regions of adult mice: the cerebral cortex (CX), subcortex (SCX), cerebellum (CB), and HC. In CX, PNGase F treatment reduces the molecular weight to approximately 116 kDa. By immunocytochemistry, affinity-purified (AP) NDCBE antibody stains the plasma membrane of neuron cell bodies and processes of rat HC neurons in primary culture as well as freshly dissociated mouse HC neurons. The AP antibody does not detect substantial NDCBE levels in freshly dissociated HC astrocytes, or astrocytes in HC or CB sections. By immunohistochemistry, the AP antibody recognizes high levels of NDCBE in neurons of CX, HC (including pyramidal neurons in Cornu Ammonis (CA)1-3 and dentate gyrus), substantial nigra, medulla, cerebellum (especially Purkinje and granular cells), and the basolateral membrane of fetal choroid plexus. Thus, NDCBE is in a position to contribute substantially to pH(i) regulation in multiple CNS neurons.

Gene Ontology Annotations Click to see Annotation Detail View

Cellular Component

Only show annotations with direct experimental evidence (0 objects hidden)

Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
basolateral plasma membrane	located_in	IDA		155230773	PMID:18359573	UniProt
plasma membrane	located_in	IDA		155230773	PMID:18359573	UniProt

Objects Annotated

Genes (Rattus norvegicus)

Slc4a8 (solute carrier family 4 member 8)

Additional Information