RGD Reference Report - Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo. - Rat Genome Database

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Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo.

Authors: Roulin, Didier  Cerantola, Yannick  Dormond-Meuwly, Anne  Demartines, Nicolas  Dormond, Olivier 
Citation: Roulin D, etal., Mol Cancer. 2010 Mar 12;9:57. doi: 10.1186/1476-4598-9-57.
RGD ID: 152995494
Pubmed: PMID:20226010   (View Abstract at PubMed)
PMCID: PMC2850884   (View Article at PubMed Central)
DOI: DOI:10.1186/1476-4598-9-57   (Journal Full-text)

The mammalian target of rapamycin (mTOR), which exists in two functionally distinct complexes, mTORC1 and mTORC2 plays an important role in tumor growth. Whereas the role of mTORC1 has been well characterized in this process, little is known about the functions of mTORC2 in cancer progression. In this study, we explored the specific role of mTORC2 in colon cancer using a short hairpin RNA expression system to silence the mTORC2-associated protein rictor. We found that downregulation of rictor in HT29 and LS174T colon cancer cells significantly reduced cell proliferation. Knockdown of rictor also resulted in a G1 arrest as observed by cell cycle analysis. We further observed that LS174T cells deficient for rictor failed to form tumors in a nude mice xenograft model. Taken together, these results show that the inhibition of mTORC2 reduces colon cancer cell proliferation in vitro and tumor xenograft formation in vivo. They also suggest that specifically targeting mTORC2 may provide a novel treatment strategy for colorectal cancer.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
colon cancer  IMP 152995494human cells in mouse modelRGD 
colon cancer  ISORICTOR (Homo sapiens)152995494; 152995494human cells in mouse modelRGD 

Objects Annotated

Genes (Rattus norvegicus)
Rictor  (RPTOR independent companion of MTOR, complex 2)

Genes (Mus musculus)
Rictor  (RPTOR independent companion of MTOR, complex 2)

Genes (Homo sapiens)
RICTOR  (RPTOR independent companion of MTOR complex 2)


Additional Information