RGD Reference Report - miR-665 promotes hepatocellular carcinoma cell migration, invasion, and proliferation by decreasing Hippo signaling through targeting PTPRB. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

miR-665 promotes hepatocellular carcinoma cell migration, invasion, and proliferation by decreasing Hippo signaling through targeting PTPRB.

Authors: Hu, Yuanchang  Yang, Chao  Yang, Shikun  Cheng, Feng  Rao, Jianhua  Wang, Xuehao 
Citation: Hu Y, etal., Cell Death Dis. 2018 Sep 20;9(10):954. doi: 10.1038/s41419-018-0978-y.
RGD ID: 151361293
Pubmed: PMID:30237408   (View Abstract at PubMed)
PMCID: PMC6148030   (View Article at PubMed Central)
DOI: DOI:10.1038/s41419-018-0978-y   (Journal Full-text)

Growing evidence suggests that aberrant microRNA (miRNA) expression contributes to hepatocellular carcinoma (HCC) development and progression. However, the potential role and mechanism of miR-665 in the progression of liver cancer remains largely unknown. Our current study showed that miR-665 expression was upregulated in HCC cells and tissues. High expression of miR-665 exhibited more severe tumor size, vascular invasion and Edmondson grading in HCC patients. Gain- or loss-of-function assays demonstrated that miR-665 promoted cell proliferation, migration, invasion, and the epithelial-mesenchymal transition (EMT) of HCC cells in vitro and in vivo. Tyrosine phosphatase receptor type B (PTPRB) was downregulated in HCC tissues, and was negatively correlated with miR-665 expression. Through western blotting and luciferase reporter assay, PTPRB was identified as a direct downstream target of miR-665. Restoration of PTPRB reverses the effects of miR-665 on HCC migration, invasion, and cell proliferation. A mechanistic study showed that PTPTRB mediated the functional role of miR-665 through regulation of the Hippo signaling pathway. In conclusion, our results suggested that miR-665 was a negative regulator of the PTPRB and could promote tumor proliferation and metastasis in HCC through decreasing Hippo signaling pathway activity, which can be a potential target for HCC treatment.



1 to 10 of 10 rows

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
MIR665Humanhepatocellular carcinoma disease_progressionIEP mRNA:increased expression:liver (human)RGD 
MIR665Humanhepatocellular carcinoma  IMP associated with metastasis and human cells in mouse modelRGD 
MIR665Humanhepatocellular carcinoma  IGIPTPRB (Homo sapiens) RGD 
Mir665Mousehepatocellular carcinoma disease_progressionISOMIR665 (Homo sapiens)mRNA:increased expression:liver (human)RGD 
Mir665Rathepatocellular carcinoma  ISOMIR665 (Homo sapiens)associated with metastasis and human cells in mouse modelRGD 
Mir665Rathepatocellular carcinoma disease_progressionISOMIR665 (Homo sapiens)mRNA:increased expression:liver (human)RGD 
Mir665Mousehepatocellular carcinoma  ISOMIR665 (Homo sapiens)associated with metastasis and human cells in mouse modelRGD 
PTPRBHumanhepatocellular carcinoma disease_progressionIEP mRNA:decreased expression:liver (human)RGD 
PtprbRathepatocellular carcinoma disease_progressionISOPTPRB (Homo sapiens)mRNA:decreased expression:liver (human)RGD 
PtprbMousehepatocellular carcinoma disease_progressionISOPTPRB (Homo sapiens)mRNA:decreased expression:liver (human)RGD 
1 to 10 of 10 rows


Genes (Rattus norvegicus)
Mir665  (microRNA 665) Ptprb  (protein tyrosine phosphatase, receptor type, B)

Genes (Mus musculus)
Mir665  (microRNA 665) Ptprb  (protein tyrosine phosphatase receptor type B)

Genes (Homo sapiens)
MIR665  (microRNA 665) PTPRB  (protein tyrosine phosphatase receptor type B)