RGD Reference Report - Cell selective induction and transcriptional activation of immediate early genes by hypoxia. - Rat Genome Database

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Cell selective induction and transcriptional activation of immediate early genes by hypoxia.

Authors: Prabhakar, N R  Shenoy, B C  Simonson, M S  Cherniack, N S 
Citation: Prabhakar NR, etal., Brain Res. 1995 Oct 30;697(1-2):266-70. doi: 10.1016/0006-8993(95)00994-2.
RGD ID: 151347626
Pubmed: PMID:8593588   (View Abstract at PubMed)
DOI: DOI:10.1016/0006-8993(95)00994-2   (Journal Full-text)

c-fos and jun belong to the immediate early response genes (IERG) that initiate phenotypic changes in response to a variety of extracellular stimuli. In the present study, we examined whether hypoxia induces IERG expression in isolated cells. Experiments were performed on pheochromocytoma-12 (PC-12), hepatoblastoma (Hep3B), neuroblastoma and fibroblast cells that were exposed either to normoxia (21% O2) or to hypoxia (5% O2) for one hour. mRNAs for c-fos, c-jun, junB, junD were analyzed by northern blot assay. Increases in IERG mRNAs were seen in PC-12, Hep3B, and fibroblasts but not in neuroblastoma cells. Significant induction of c-fos mRNA was seen with hypoxic exposure as short as 15 min and the effects persisted at 10 h of low pO2 exposure. Hypoxia stimulated transcription from a 356 bp fragment of the c-fos promoter linked to a choloramphenicol acetyl transferase reporter in PC-12 but not in neuroblastoma cells. Fetal bovine serum, however, activated c-fos promoter both in PC-12 and neuroblastoma cells. These results demonstrate cell type selective mechanisms for c-fos promoter activation that require nucleic acid sequences with in the first 356 bp of the c-fos promoter. These observations suggest that increased IERG transcription is one of the early events in genomic adaptations to hypoxia.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FosRatcellular response to hypoxia  IEP  RGD 
JunRatcellular response to hypoxia  IEP  RGD 
JunbRatcellular response to hypoxia  IEP  RGD 
JundRatcellular response to hypoxia  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fos  (Fos proto-oncogene, AP-1 transcription factor subunit)
Jun  (Jun proto-oncogene, AP-1 transcription factor subunit)
Junb  (JunB proto-oncogene, AP-1 transcription factor subunit)
Jund  (JunD proto-oncogene, AP-1 transcription factor subunit)


Additional Information