RGD Reference Report - Pseudomonas aeruginosa colonization enhances ventilator-associated pneumonia-induced lung injury. - Rat Genome Database

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Pseudomonas aeruginosa colonization enhances ventilator-associated pneumonia-induced lung injury.

Authors: Tsay, Tzyy-Bin  Jiang, Yu-Zhen  Hsu, Ching-Mei  Chen, Lee-Wei 
Citation: Tsay TB, etal., Respir Res. 2016 Aug 9;17(1):101. doi: 10.1186/s12931-016-0417-5.
RGD ID: 150340682
Pubmed: PMID:27506464   (View Abstract at PubMed)
PMCID: PMC4979138   (View Article at PubMed Central)
DOI: DOI:10.1186/s12931-016-0417-5   (Journal Full-text)


BACKGROUND: Pseudomonas aeruginosa (PA) is the single-most common pathogen of ventilator-associated pneumonia (VAP). Large quantities of PA in the trachea of ventilated patients are associated with an increased risk of death. However, the role of PA colonization in PA VAP-induced lung injury remains elusive. This study examined the effect and mechanism of PA colonization in VAP-induced lung injury.
METHODS: C57BL/6 wild-type (WT) and c-Jun N-terminal kinase knockout (JNK1(-/-)) mice received mechanical ventilation for 3 h at 2 days after receiving nasal instillation of PA (1 × 10(6) colony forming unit) or normal saline.
RESULTS: Intranasal instillation of PA or mechanical ventilation induced the expression of interleukin-6 (IL-6) in the lungs. Phospho-JNK protein expression in the lungs was significantly increased in mice receiving mechanical ventilation after PA instillation as compared with those receiving ventilation alone. Mechanical ventilation after PA instillation significantly increased the expression of tumor necrosis factor-α (TNF-α), IL-1β, and macrophage inflammatory protein-2 (MIP-2) proteins; neutrophil sequestration; and TNF-α, IL-1β, and IL-6 levels in the lungs of WT mice, but not in JNK1(-/-) mice.
CONCLUSION: PA colonization plays an important role in PA VAP-induced lung injury through the induction of JNK1-mediated inflammation. PA-induced VAP causes lung injury through JNK signaling pathway in the lungs. JNK inhibition in ICU patients with higher percentages of PA colonization may reduce VAP-induced lung injury and mortality.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MAPK8HumanPseudomonas Infections amelioratesISOMapk8 (Mus musculus)associated with Pneumonia and Ventilator-AssociatedRGD 
Mapk8RatPseudomonas Infections amelioratesISOMapk8 (Mus musculus)associated with Pneumonia and Ventilator-AssociatedRGD 
Mapk8MousePseudomonas Infections amelioratesIMP associated with Pneumonia and Ventilator-AssociatedRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mapk8  (mitogen-activated protein kinase 8)

Genes (Mus musculus)
Mapk8  (mitogen-activated protein kinase 8)

Genes (Homo sapiens)
MAPK8  (mitogen-activated protein kinase 8)


Additional Information