RGD Reference Report - Tri-iodothyronine upregulates adiponutrin mRNA expression in rat and human adipocytes. - Rat Genome Database

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Tri-iodothyronine upregulates adiponutrin mRNA expression in rat and human adipocytes.

Authors: Calvo, R M  Obregon, M J 
Citation: Calvo RM and Obregon MJ, Mol Cell Endocrinol. 2009 Nov 13;311(1-2):39-46. doi: 10.1016/j.mce.2009.07.006. Epub 2009 Jul 18.
RGD ID: 14985223
Pubmed: PMID:19619606   (View Abstract at PubMed)
DOI: DOI:10.1016/j.mce.2009.07.006   (Journal Full-text)

Adiponutrin (PNPLA3) is expressed in adipose tissue. Although its precise function is unknown, some data suggest a dual role in lipid homeostasis. We have investigated the influence of thyroid hormone (TH) on PNPLA3 mRNA, in rat and human cultured white adipocytes and in rat white adipose tissue (WAT). Pnpla3 mRNA increased during differentiation of rat adipocytes in an insulin-dependent manner. Tri-iodothyronine further increased Pnpla3 expression at any day during differentiation and its effects were time and dose-dependent. The Pnpla3 mRNA half-life was stabilized by tri-iodothyronine, but a transcriptional component was also observed. Pnpla3 mRNA decreased in WAT of hypothyroid rats and was partially restored by treatment with TH. Taqman analysis showed that tri-iodothyronine also increased human PNPLA3 expression in cultured subcutaneous adipocytes from obese patients. In conclusion, PNPLA3 mRNA expression is upregulated by tri-iodothyronine in adipocytes in vitro, in humans and rats, and in vivo in rat WAT.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hypothyroidism treatmentISOPnpla3 (Rattus norvegicus)14985223; 14985223 RGD 
hypothyroidism treatmentIEP 14985223 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to 3,3',5-triiodo-L-thyronine  IEP 14985223 RGD 
cellular response to insulin stimulus  IEP 14985223 RGD 
white fat cell differentiation  IEP 14985223 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pnpla3  (patatin-like phospholipase domain containing 3)

Genes (Mus musculus)
Pnpla3  (patatin-like phospholipase domain containing 3)

Genes (Homo sapiens)
PNPLA3  (patatin like phospholipase domain containing 3)


Additional Information