RGD Reference Report - Regulation of Apoptosis by miR-122 in Pterygium via Targeting Bcl-w. - Rat Genome Database

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Regulation of Apoptosis by miR-122 in Pterygium via Targeting Bcl-w.

Authors: Cui, Yu-Hong  Li, Hong-Yang  Gao, Zi-Xun  Liang, Na  Ma, Sheng-Sheng  Meng, Fan-Jian  Li, Zhi-Jie  Pan, Hong-Wei 
Citation: Cui YH, etal., Invest Ophthalmol Vis Sci. 2016 Jul 1;57(8):3723-30. doi: 10.1167/iovs.16-19402.
RGD ID: 14394423
Pubmed: PMID:27415790   (View Abstract at PubMed)
DOI: DOI:10.1167/iovs.16-19402   (Journal Full-text)


PURPOSE: To identify the differently expressed micro (mi) RNAs in pterygium compared with normal conjunctiva and investigate the potential role of miRNAs in the pathogenesis of pterygium.
METHODS: With microRNA microarray and quantitative RT-PCR, we identified that microRNA-122 (miR-122) was significantly decreased in pterygium tissue. We detected the expression of Bcl-w, a predicted target of miR-122, in both pterygium and normal conjunctiva, as well as its correlation with the expression of miR-122. Pterygium epithelial cells were isolated and cultured, and transfected with miR-122 mimic or miR-122 inhibitor to change the miR-122 levels. The regulation of Bcl-w expression by miR-122 was examined with luciferase activity assay, quantitative (q) RT-PCR, and Western blot. The effect of the miR-122 on the apoptosis of cultured pterygium epithelial cells was investigated with TUNEL staining and caspase activity assay.
RESULTS: We found the expression of Bcl-w, with an inverse correlation with the expression of miR-122, was significantly increased in pterygium, especially in the superficial layer of epithelium. In cultured pterygium epithelial cells, miR-122 could specifically combine with Bcl-w mRNA, and negatively regulated the expression of Bcl-w. Suppression of miR-122 could reduce apoptosis and caspase activity in pterygium epithelial cell treated with TNFα/cycloheximide (CHX), and this effect was abolished by inhibition of the expression of Bcl-w with specific siRNA.
CONCLUSIONS: Decreased expression of miR-122 in pterygium might result in abnormal cell apoptosis via its regulation of the expression of Bcl-w, and subsequently contribute to the development of pterygium.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
pterygium  ISOBcl2l2 (Rattus norvegicus)14394423; 14394423RNA:increased expression:conjunctivaRGD 
pterygium  IEP 14394423RNA:increased expression:conjunctivaRGD 
pterygium  ISOMir122 (Rattus norvegicus)14394423; 14394423RNA:decreased expression:conjunctivaRGD 
pterygium  IEP 14394423RNA:decreased expression:conjunctivaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Bcl2l2  (Bcl2-like 2)
Mir122  (microRNA 122)

Genes (Mus musculus)
Bcl2l2  (BCL2-like 2)
Mir122  (microRNA 122)

Genes (Homo sapiens)
BCL2L2  (BCL2 like 2)
MIR122  (microRNA 122)


Additional Information