RGD Reference Report - Renal neuronal nitric oxide synthase protein expression as a marker of renal injury. - Rat Genome Database

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Renal neuronal nitric oxide synthase protein expression as a marker of renal injury.

Authors: Szabo, Attila J  Wagner, Laszlo  Erdely, Aaron  Lau, Kim  Baylis, Chris 
Citation: Szabo AJ, etal., Kidney Int. 2003 Nov;64(5):1765-71. doi: 10.1046/j.1523-1755.2003.00260.x.
RGD ID: 13825139
Pubmed: PMID:14531809   (View Abstract at PubMed)
DOI: DOI:10.1046/j.1523-1755.2003.00260.x   (Journal Full-text)


BACKGROUND: Animal studies suggest that nitric oxide deficiency occurs in the remnant after 5/6 removal of renal mass. The present studies investigated the time course in relation to progression of renal disease, as well as the impact on individual renal nitric oxide synthase (NOS) isoforms.
METHODS: Rats were studied from 2 to 11 weeks after 5/6 ablation/infarction (A/I) of renal mass, with acceleration of progression by high protein and salt intake, in some groups. Measurements were made before sacrifice of 24-hour protein and creatinine excretion, blood was taken for creatinine and blood urea nitrogen (BUN) determination and the kidneys were investigated histologically for structural damage, abundance of endothelial NOS (eNOS) and neuronal NOS (nNOS), and in some groups for in vitro NOS activity.
RESULTS: A time-dependent fall in glomerular filtration rate (GFR) and rise in proteinuria and glomerular sclerosis developed after 5/6 A/I. The nNOS abundance in cortex and medulla was decreased relative to shams, in all but the mildest injury and there was a strong, steep correlation between level of glomerular sclerosis and the degree of reduction in renal nNOS. Where measured, cortical NOS activity correlated with the nNOS abundance. In contrast, the eNOS abundance was either increased or unchanged in rats post A/I.
CONCLUSION: Renal nNOS abundance was reduced in the 5/6 A/I model of renal disease when plasma creatinine> approximately 1 mg/dL and when> approximately 20% of remaining glomeruli were sclerosed.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NOS1Humanacute kidney failure  ISONos1 (Rattus norvegicus) RGD 
Nos1Ratacute kidney failure  IEP  RGD 
Nos1Mouseacute kidney failure  ISONos1 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nos1  (nitric oxide synthase 1)

Genes (Mus musculus)
Nos1  (nitric oxide synthase 1, neuronal)

Genes (Homo sapiens)
NOS1  (nitric oxide synthase 1)


Additional Information