RGD Reference Report - CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.

Authors: Miyazaki, Kazufumi  Ohno, Koji  Tamura, Naoaki  Sasaki, Takeshi  Sato, Kohji 
Citation: Miyazaki K, etal., Exp Cell Res. 2012 Aug 15;318(14):1716-25. doi: 10.1016/j.yexcr.2012.05.006. Epub 2012 May 30.
RGD ID: 13432282
Pubmed: PMID:22659164   (View Abstract at PubMed)
DOI: DOI:10.1016/j.yexcr.2012.05.006   (Journal Full-text)

CLP36 is a member of the ALP/Enigma protein family and has been shown to be localized to stress fibers in various cells. We previously reported that depletion of CLP36 caused loss of stress fibers in BeWo choriocarcinoma cells, but it remains unclear how CLP36 contributes to stress fiber formation. In this study, we generated CLP36-depleted F2408 fibroblasts and found that stress fibers showed abnormal non-oriented organization in these cells. In addition to CLP36, F2408 cells contained RIL, another ALP/Enigma protein, and we demonstrated that RIL could compensate for the role of CLP36 in stress fiber formation. CLP36 and RIL form a complex with α-actinin-1 and palladin. We found a strong correlation between loss of CLP36/RIL and failure of α-actinin-1 or palladin to localize on stress fibers. In addition, time lapse observation revealed that incorporation of RIL stabilizes stress fibers and that CLP36 influences the dynamic architecture of these fibers. Our findings indicate that CLP36 and RIL have a redundant role in the formation of stress fibers, but have different effects on stress fiber dynamics in F2408 cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pdlim1Ratactin cytoskeleton organization involved_inIMP PMID:22659164UniProt 
Pdlim1Ratestablishment or maintenance of actin cytoskeleton polarity involved_inIMP PMID:22659164UniProt 
Pdlim1Ratfibroblast migration involved_inIMP PMID:22659164UniProt 
Pdlim1Ratmaintenance of cell polarity involved_inIMP PMID:22659164UniProt 
Pdlim4Ratpositive regulation of stress fiber assembly involved_inIMP PMID:22659164UniProt 
Pdlim1Ratstress fiber assembly involved_inIMP PMID:22659164UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pdlim1Ratactin cytoskeleton located_inIDA PMID:22659164UniProt 
Pdlim1Ratcytoplasm located_inIDA PMID:22659164UniProt 
PalldRatstress fiber located_inIDA PMID:22659164UniProt 
Pdlim1Ratstress fiber located_inIDA PMID:22659164UniProt 
Pdlim4Ratstress fiber located_inIDA PMID:22659164UniProt 
Pdlim1RatZ disc located_inIDA PMID:22659164UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pdlim1Ratactin binding enablesIDA PMID:22659164UniProt 
Actn1Ratprotein binding enablesIPIUniProtKB:P52944PMID:22659164UniProt 
Pdlim1Ratprotein binding enablesIPIUniProtKB:Q9Z1P2PMID:22659164UniProt 
Pdlim1Ratprotein binding enablesIPIUniProtKB:P36202PMID:22659164UniProt 
Pdlim4Ratprotein binding enablesIPIUniProtKB:P52944PMID:22659164UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Actn1  (actinin, alpha 1)
Palld  (palladin, cytoskeletal associated protein)
Pdlim1  (PDZ and LIM domain 1)
Pdlim4  (PDZ and LIM domain 4)


Additional Information