RGD Reference Report - Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton. - Rat Genome Database

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Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton.

Authors: Terry-Lorenzo, RT  Elliot, E  Weiser, DC  Prickett, TD  Brautigan, DL  Shenolikar, S 
Citation: Terry-Lorenzo RT, etal., J Biol Chem 2002 Nov 29;277(48):46535-43. Epub 2002 Sep 21.
RGD ID: 1299366
Pubmed: PMID:12270929   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M206960200   (Journal Full-text)

Inhibitor-2 (I-2) bound protein phosphatase-1 (PP1) and several PP1-binding proteins from rat brain extracts, including the actin-binding proteins, neurabin I and neurabin II. Neurabins from rat brain lysates were sedimented by I-2 and its structural homologue, I-4. The central domain of both neurabins bound PP1 and I-2, and mutation of a conserved PP1-binding motif abolished neurabin binding to both proteins. Microcystin-LR, a PP1 inhibitor, also attenuated I-2 binding to neurabins. Immunoprecipitation of neurabin I established its association with PP1 and I-2 in HEK293T cells and suggested that PP1 mediated I-2 binding to neurabins. The C terminus of I-2, although not required for PP1 binding, facilitated PP1 recruitment by neurabins, which also targeted I-2 to polymerized F-actin. Mutations that attenuated PP1 binding to I-2 and neurabin I suggested distinct and overlapping sites for these two proteins on the PP1 catalytic subunit. Immunocytochemistry in epithelial cells and cultured hippocampal neurons showed that endogenous neurabin II and I-2 colocalized at actin-rich structures, consistent with the ability of neurabins to target the PP1.I-2 complex to actin cytoskeleton and regulate cell morphology.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppp1r2Ratnegative regulation of protein binding  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppp1r2Ratdendritic spine  IDA  RGD 
Ppp1r2Ratgrowth cone  IDA  RGD 
Ppp1r9bRatgrowth cone  IDA  RGD 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Ppp1cc  (protein phosphatase 1 catalytic subunit gamma)
Ppp1r2  (protein phosphatase 1, regulatory (inhibitor) subunit 2)
Ppp1r9a  (protein phosphatase 1, regulatory subunit 9A)
Ppp1r9b  (protein phosphatase 1, regulatory subunit 9B)

Objects referenced in this article
Gene PPP1R2 protein phosphatase 1 regulatory inhibitor subunit 2 Homo sapiens

Additional Information