RGD Reference Report - Lipocalin 2 as a new biomarker for fetal lung hypoplasia in congenital diaphragmatic hernia. - Rat Genome Database

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Lipocalin 2 as a new biomarker for fetal lung hypoplasia in congenital diaphragmatic hernia.

Authors: Tsuda, Hiroyuki  Kotani, Tomomi  Nakano, Tomoko  Imai, Kenji  Hirako, Shima  Li, Hua  Kikkawa, Fumitaka 
Citation: Tsuda H, etal., Clin Chim Acta. 2016 Nov 1;462:71-76. doi: 10.1016/j.cca.2016.08.023. Epub 2016 Aug 31.
RGD ID: 126790533
Pubmed: PMID:27592368   (View Abstract at PubMed)
DOI: DOI:10.1016/j.cca.2016.08.023   (Journal Full-text)


BACKGROUND: Congenital diaphragmatic hernia (CDH) causes pulmonary hypoplasia, which are often fatal. We established a new biomarker for fetal lung hypoplasia in CDH.
METHODS: We collected newborn lung tissue specimens at E21 from normal and nitrofen-induced CDH rats (administered 100mg orally at E9) and performed a microarray analysis and real-time PCR (RT-PCR). Sixty-three human amniotic fluid (AF) samples, including samples from isolated CDH cases (n=33) and Cesarean section (CS) cases without fetal complications (controls) (n=30), were obtained. All AF samples were obtained at the time of CS, which was performed after 35-38 gestational weeks, from April 2007 to January 2016.
RESULTS: A microarray analysis and RT-PCR showed decreased gene expression levels of lipocalin 2 (LCN2) in the nitrofen-induced CDH lungs (p<0.05). We next examined the LCN2 levels in human AF samples using ELISA and the levels were significantly lower in the CDH cases than in controls (73.7ng/ml vs 163.8ng/ml; p<0.05). A significant positive correlation was observed between the amniotic LCN2 level and the observed/expected lung-to-head ratio (p<0.001).
CONCLUSIONS: LCN2 may be a potentially useful biomarker for lung hypoplasia in a rat and human CDH.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LCN2Humancongenital diaphragmatic hernia  ISOLcn2 (Rattus norvegicus)mRNA:decreased expression:lungRGD 
LCN2Humancongenital diaphragmatic hernia  IEP protein:decreased expression:amniotic fluidRGD 
Lcn2Ratcongenital diaphragmatic hernia  ISOLCN2 (Homo sapiens)protein:decreased expression:amniotic fluidRGD 
Lcn2Ratcongenital diaphragmatic hernia  IEP mRNA:decreased expression:lungRGD 
Lcn2Mousecongenital diaphragmatic hernia  ISOLcn2 (Rattus norvegicus)mRNA:decreased expression:lungRGD 
Lcn2Mousecongenital diaphragmatic hernia  ISOLCN2 (Homo sapiens)protein:decreased expression:amniotic fluidRGD 

Objects Annotated

Genes (Rattus norvegicus)
Lcn2  (lipocalin 2)

Genes (Mus musculus)
Lcn2  (lipocalin 2)

Genes (Homo sapiens)
LCN2  (lipocalin 2)


Additional Information