RGD Reference Report - TGF-beta1-induced Fascin1 promotes cell invasion and metastasis of human 786-0 renal carcinoma cells. - Rat Genome Database

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TGF-beta1-induced Fascin1 promotes cell invasion and metastasis of human 786-0 renal carcinoma cells.

Authors: Huang, W  Cen, S  Kang, XL  Wang, WF  Wang, Y  Chen, X 
Citation: Huang W, etal., Acta Histochem. 2016 Mar;118(2):144-51. doi: 10.1016/j.acthis.2015.12.005. Epub 2015 Dec 31.
RGD ID: 11560966
Pubmed: PMID:26749573   (View Abstract at PubMed)
DOI: DOI:10.1016/j.acthis.2015.12.005   (Journal Full-text)

OBJECTIVE: To investigate the effect of transforming growth factor-beta1 (TGF-beta1) on the expression of Fascin1 protein and its impact on cell invasion and metastasis in human renal carcinoma. METHODS: Renal tissue slices of 52 cases when undergoing radical nephrectomy were collected to be the observation group, and the normal renal tissues of 23 cases when undergoing nephrectomy due to trauma were collected to be the control group. The expressions of TGF-beta1 and Fascin1 were measured by immunohistochemical staining. Human renal carcinoma 786-0 cell line was selected as the study subject. The cells were divided into six groups including NT (no transfection), si-NC (transfection with pGenesil-1-con) si-Fascin1 (transfection with pGen-1-FSCN1) groups, and three corresponding groups: NT, si-NC and si-Fascin1 groups treated with TGF-beta1. RT-qPCR, Western-Blot, Transwell, and flow cytometry method were used in this study. RESULTS: The expressions of TGF-beta1 and Fascin1 in the observation group were significantly higher than those in the control group. The expression of TGF-beta1 was positively correlated with that of Fascin1. After 24 and 48h of treatment with TGF-beta1 (10ng/mL), the invasive and metastatic abilities of the 786-0 cells in the NT and si-NC groups were higher than those before the treatment (P<0.05). Comparing the three groups before TGF-beta1 treatment, the invasive and metastatic ability of 786-0 cells in the si-Fascin1 were significantly lower than those in the NT group and si-NC group (P<0.05). CONCLUSION: TGF-beta1 could induce the expressions of 786-0 Fascin1 mRNA and protein and thus improve the invasive and metastatic ability of human 786-0 renal carcinoma cell.




  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
TGFB1Humanrenal cell carcinoma  IEP  RGD 
Tgfb1Ratrenal cell carcinoma  ISOTGFB1 (Homo sapiens) RGD 
Tgfb1Mouserenal cell carcinoma  ISOTGFB1 (Homo sapiens) RGD 


Genes (Rattus norvegicus)
Tgfb1  (transforming growth factor, beta 1)

Genes (Mus musculus)
Tgfb1  (transforming growth factor, beta 1)

Genes (Homo sapiens)
TGFB1  (transforming growth factor beta 1)