RGD Reference Report - PRAME is a membrane and cytoplasmic protein aberrantly expressed in chronic lymphocytic leukemia and mantle cell lymphoma. - Rat Genome Database

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PRAME is a membrane and cytoplasmic protein aberrantly expressed in chronic lymphocytic leukemia and mantle cell lymphoma.

Authors: Proto-Siqueira, R  Figueiredo-Pontes, LL  Panepucci, RA  Garcia, AB  Rizzatti, EG  Nascimento, FM  Ishikawa, HC  Larson, RE  Falcao, RP  Simpson, AJ  Gout, I  Filonenko, V  Rego, EM  Zago, MA 
Citation: Proto-Siqueira R, etal., Leuk Res. 2006 Nov;30(11):1333-9. Epub 2006 Apr 18.
RGD ID: 11535021
Pubmed: PMID:16620968   (View Abstract at PubMed)
DOI: DOI:10.1016/j.leukres.2006.02.031   (Journal Full-text)

The preferentially expressed antigen in melanoma (PRAME) gene is aberrantly expressed in chronic lymphoproliferative disorders (CLD). We produced and characterized an anti-PRAME monoclonal antibody (MoAb), which was then applied in a quantitative flow cytometric (QFC) method to evaluate PRAME expression in leukemic cells from the peripheral blood (PB) of 47 patients with chronic lymphocytic leukemia and seven with mantle cell lymphoma as well as in the PB mononuclear cells (PBMCs) and B lymphocytes from 15 healthy subjects. Approximately 90% of CLD, but none of the normal samples, presented more than 20% of PRAME+ lymphocytes. Moreover, the intensity of PRAME expression was significantly higher in CLD cells compared to normal B lymphocytes and PBMCs. By immunofluorescence microscopy and by permeabilized flow cytometry we demonstrated that PRAME is a membrane antigen and a cytoplasmic protein aberrantly expressed in malignant CLD. Our results suggest that the analysis of PRAME protein may contribute for the distinction between normal and leukemic cells in CLD, and that PRAME may be a potential target for therapy.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
B-Cell Chronic Lymphocytic Leukemia  IEP 11535021protein:increased expression:mononuclear cell (human)RGD 
B-Cell Chronic Lymphocytic Leukemia  ISOPRAME (Homo sapiens)11535021; 11535021protein:increased expression:mononuclear cell (human)RGD 
mantle cell lymphoma  IEP 11535021protein:increased expression:mononuclear cell (human)RGD 
mantle cell lymphoma  ISOPRAME (Homo sapiens)11535021; 11535021protein:increased expression:mononuclear cell (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pramex1  (PRAME like, X-linked 1)

Genes (Mus musculus)
Pramex1  (PRAME like, X-linked 1)

Genes (Homo sapiens)
PRAME  (PRAME nuclear receptor transcriptional regulator)


Additional Information