RGD Reference Report - Genomewide analysis of DNA methylation in rat lungs with lipopolysaccharideinduced acute lung injury. - Rat Genome Database

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Genomewide analysis of DNA methylation in rat lungs with lipopolysaccharideinduced acute lung injury.

Authors: Zhang, XQ  Lv, CJ  Liu, XY  Hao, D  Qin, J  Tian, HH  Li, Y  Wang, XZ 
Citation: Zhang XQ, etal., Mol Med Rep. 2013 May;7(5):1417-24. doi: 10.3892/mmr.2013.1405. Epub 2013 Mar 28.
RGD ID: 11533951
Pubmed: PMID:23546543   (View Abstract at PubMed)
DOI: DOI:10.3892/mmr.2013.1405   (Journal Full-text)

Acute lung injury and acute respiratory distress syndrome (ALI/ARDS) are associated with high morbidity and mortality in patients, however, the precise pathogenesis of ALI/ARDS remains unknown. Lipopolysaccharide (LPS) exhibits a number of critical functions and may be associated with the DNA methylation of genes in the lungs. In the present study a genomewide analysis of DNA methylation was performed in rat lungs with LPSinduced ALI/ARDS. Normal and LPSinduced lung tissues with ALI were analyzed using methylated DNA immunoprecipitation and a rat DNA methylation promoter plus CpG island microarray and the candidate genes were validated by quantitative reverse transcriptase polymerase chain reaction (qRTPCR). Aberrant DNA methylation of the promoter regions of 1,721 genes and the CpG islands of 990 genes was identified when normal lung tissues and lung tissues with LPSinduced ALI/ARDS were compared. These genes were commonly located on chromosomes 1, 3, 5, 7 and 10 (P<0.01). Methylation level and CpG density were compared and it was found that genes associated with high CpG density promoters had a high ratio of methylation. Furthermore, we performed gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In addition, three genes (Mapk3, Pak1 and Rac2) were validated in the control and lung tissues with ALI by RTPCR. The results indicate that aberrant DNA methylation of lung tissues may be involved in the pathophysiology of LPSinduced ALI/ARDS. Future studies are required to evaluate the therapeutic and prognostic value of the current novel observations in ALI/ARDS.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PAK1HumanAcute Lung Injury  ISOPak1 (Rattus norvegicus)DNA:hypermethylationRGD 
Pak1RatAcute Lung Injury  IDA DNA:hypermethylationRGD 
Pak1MouseAcute Lung Injury  ISOPak1 (Rattus norvegicus)DNA:hypermethylationRGD 

Objects Annotated

Genes (Rattus norvegicus)
Pak1  (p21 (RAC1) activated kinase 1)

Genes (Mus musculus)
Pak1  (p21 (RAC1) activated kinase 1)

Genes (Homo sapiens)
PAK1  (p21 (RAC1) activated kinase 1)


Additional Information