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[Regulatory mechanisms of PI3K/AKT signaling pathway in acute leukemia].

Authors: Wang, WL  Zhang, YC  Zeng, HM  Hua, CL  Wei, W  Xu, J  Zhu, XF  Cheng, T  Yuan, WP 
Citation: Wang WL, etal., Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Feb;20(1):18-21.
Pubmed: (View Article at PubMed) PMID:22391157

This study was aimed to analyze the expression profiles of PI3K/AKT signaling pathway genes from bone marrow samples of AML and ALL patients and normal samples. AML, ALL and normal bone marrow samples were collected from 6 AML, 6 ALL patients and 4 normal persons. The expression of PI3K/AKT signaling pathway genes including PTEN, CCND1, mTOR, RICTOR, FOXO1 were detected by real-time fluorescent quantification RT-PCR while GAPDH gene expression was used as an internal reference. The relative gene expression level was calculated by the method of the 2(-DeltaDeltaCt). The results showed that the gene expression profiles were different between normal and leukemic groups. PTEN, mTOR and RICTOR expression levels were down-regulated, while FOXO1 and CCND1 levels were up-regulated in AML and ALL. PTEN was down-regulated in 10 out of the 12 samples; mTOR was down-regulated in 9 out of the 12 samples; RICTOR was down-regulated in 7 out of the 12 samples; FOXO1 was up-regulated in 9 out of the 12 samples and CCND1 was up-regulated in 7 out of the 12 samples. It is concluded that PI3K/AKT signal pathway is activated in both AML and ALL leukemic cells.


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RGD Object Information
RGD ID: 11352824
Created: 2016-07-20
Species: All species
Last Modified: 2016-07-20
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.