RGD Reference Report - Sodium butyrate protects against severe burn-induced remote acute lung injury in rats. - Rat Genome Database

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Sodium butyrate protects against severe burn-induced remote acute lung injury in rats.

Authors: Liang, X  Wang, RS  Wang, F  Liu, S  Guo, F  Sun, L  Wang, YJ  Sun, YX  Chen, XL 
Citation: Liang X, etal., PLoS One. 2013 Jul 11;8(7):e68786. doi: 10.1371/journal.pone.0068786. Print 2013.
RGD ID: 10402098
Pubmed: PMID:23874764   (View Abstract at PubMed)
PMCID: PMC3708909   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0068786   (Journal Full-text)

High-mobility group box 1 protein (HMGB1), a ubiquitous nuclear protein, drives proinflammatory responses when released extracellularly. It plays a key role as a distal mediator in the development of acute lung injury (ALI). Sodium butyrate, an inhibitor of histone deacetylase, has been demonstrated to inhibit HMGB1 expression. This study investigates the effect of sodium butyrate on burn-induced lung injury. Sprague-Dawley rats were divided into three groups: 1) sham group, sham burn treatment; 2) burn group, third-degree burns over 30% total body surface area (TBSA) with lactated Ringer's solution for resuscitation; 3) burn plus sodium butyrate group, third-degree burns over 30% TBSA with lactated Ringer's solution containing sodium butyrate for resuscitation. The burned animals were sacrificed at 12, 24, and 48 h after burn injury. Lung injury was assessed in terms of histologic changes and wet weight to dry weight (W/D) ratio. Tumor necrosis factor (TNF)-alpha and interleukin (IL)-8 protein concentrations in bronchoalveolar lavage fluid (BALF) and serum were measured by enzyme-linked immunosorbent assay, and HMGB1 expression in the lung was determined by Western blot analysis. Pulmonary myeloperoxidase (MPO) activity and malondialdehyde (MDA) concentration were measured to reflect neutrophil infiltration and oxidative stress in the lung, respectively. As a result, sodium butyrate significantly inhibited the HMGB1 expressions in the lungs, reduced the lung W/D ratio, and improved the pulmonary histologic changes induced by burn trauma. Furthermore, sodium butyrate administration decreased the TNF-alpha and IL-8 concentrations in BALF and serum, suppressed MPO activity, and reduced the MDA content in the lungs after severe burn. These results suggest that sodium butyrate attenuates inflammatory responses, neutrophil infiltration, and oxidative stress in the lungs, and protects against remote ALI induced by severe burn, which is associated with inhibiting HMGB1 expression.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HMGB1HumanAcute Lung Injury treatmentISOHmgb1 (Rattus norvegicus)associated with BurnsRGD 
Hmgb1RatAcute Lung Injury treatmentIEP associated with BurnsRGD 
Hmgb1MouseAcute Lung Injury treatmentISOHmgb1 (Rattus norvegicus)associated with BurnsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Hmgb1  (high mobility group box 1)

Genes (Mus musculus)
Hmgb1  (high mobility group box 1)

Genes (Homo sapiens)
HMGB1  (high mobility group box 1)


Additional Information