RGD Reference Report - Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-kappaB p65 signal pathway. - Rat Genome Database

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Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-kappaB p65 signal pathway.

Authors: Li, YW  Zhang, Y  Zhang, L  Li, X  Yu, JB  Zhang, HT  Tan, BB  Jiang, LH  Wang, YX  Liang, Y  Zhang, XS  Wang, WS  Liu, HG 
Citation: Li YW, etal., Gene. 2014 May 25;542(1):46-51. doi: 10.1016/j.gene.2014.03.021. Epub 2014 Mar 12.
RGD ID: 10401866
Pubmed: PMID:24630969   (View Abstract at PubMed)
DOI: DOI:10.1016/j.gene.2014.03.021   (Journal Full-text)

Tea polyphenols (TP) was investigated in rats for its protective effect on renal ischemia/reperfusion injury (RIRI). Rats were randomized into groups as follows: (I) sham group (n=10); (II) RIRI group (n=10); (III) RIRI+TP (100mg/kg) group (n=5); (IV) RIRI+TP (200mg/kg) group (n=5); (V) RIRI+TP+ Astragalus mongholicus aqueous extract (AMAE) (300 mg/kg+100mg/kg) group (n=5). For the IRI+TP groups, rats were orally given with tea polyphenols (100, 200 and 300 mg/kg body weight) once daily 10 days before induction of ischemia, followed by renal IRI. For the sham group and RIRI group, rats were orally given with equal volume of saline once daily 10 days before induction of ischemia, followed by renal IRI. Results showed that tea polyphenol pretreatment significantly suppressed ROS level and MDA release. On the other hand, in rats subjected to ischemia-reperfusion, the activities of endogenous antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GSH-Px) showed recovery, whereas the levels of urea nitrogen and serum creatinine were reduced by administration of tea polyphenols orally for 10 days prior to ischemia-reperfusion. Moreover, tea polyphenol pretreatment significantly decreased TLR4 and NF-kappaB p65 protein expression levels in RIRI rats. At the same time, tea polyphenol pretreatment attenuated the increased level of serum IL-1beta, IL-6, ICAM-1 and TNF-alpha, and enhanced IL-10 production in RIRI rats. Furthermore, tea polyphenol pretreatment significantly decreased renal epithelial tubular cell apoptosis induced by renal ischemia/reperfusion, alleviating renal ischemia/reperfusion injury. These results cumulatively indicate that tea polyphenol pretreatment could suppress the TLR4/NF-kappaB p65 signaling pathway, protecting renal tubular epithelial cells against ischemia/reperfusion-induced apoptosis, which implies that antioxidants may be a potential and effective agent for prevention of the ischemic/reperfusion injury through the suppression extrinsic apoptotic signal pathway induced by TLR4/NF-kappaB p65 signal pathway. Moreover, supplement of AMAE can increased renal protection effect of TP.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GSRHumanKidney Reperfusion Injury treatmentISOGsr (Rattus norvegicus) RGD 
GsrRatKidney Reperfusion Injury treatmentIEP  RGD 
GsrMouseKidney Reperfusion Injury treatmentISOGsr (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gsr  (glutathione-disulfide reductase)

Genes (Mus musculus)
Gsr  (glutathione reductase)

Genes (Homo sapiens)
GSR  (glutathione-disulfide reductase)


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