RGD Reference Report - [Effects of prednisone on renal FAK and Pyk2 expressions in rats with adriamycin- induced nephritis]. - Rat Genome Database

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[Effects of prednisone on renal FAK and Pyk2 expressions in rats with adriamycin- induced nephritis].

Authors: Chen, X  Wang, J  Zheng, J  Wu, X  Liu, C  Lin, X 
Citation: Chen X, etal., Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jul;34(8):1149-53.
RGD ID: 10041073
Pubmed: PMID:25176084   (View Abstract at PubMed)

OBJECTIVE: To investigate the effects of prednisone on the expressions of FAK and Pyk2 in the kidneys of rats with adriamycin-induced nephritis. METHODS: Thirty SD rats were randomized into normal control group, adriamycin-induced nephritic model group, and prednisone treatment group (n=10). Prednisone was administered at 10 mg/kg once daily in nephritic rats starting since the 7th day after adriamycin injection. Twenty-four-hour proteinuria was measured in the rats at different time points, and renal tissue histology was examined using transmission electron microscope. The expression levels of Pyk2, FAK and nephrin mRNA in the renal tissue were detected tested by RT-PCR, and the protein expressions of FAK, Pyk2, phosphorylated Pyk2 and phosphorylated FAK-Tyr397 were detected by Western blotting; immunohistochemistry was used for detecting nephrin protein expression in the kidney. RESULTS: Compared with the normal control group, the rats with adriamycin-induced nephritis showed significantly increased proteinuria (P<0.01), which was obviously lowered by prednisone treatment (P<0.01). Transmission electron microscopy revealed extensive fusion of the foot processes of the podocytes in the model group. Prednisone treatment promoted nephrin expression in the kidney (P<0.05). Compared with the control group, the model and prednisone treated groups showed significantly lowered nephrin mRNA expression (P<0.01) but increased FAK mRNA expression (P<0.01), but prednisone-treated group had a higher nephrin mRNA expression than the model group (P<0.05). The model group exhibited significantly increased expressions of FAK total and phosphorylated proteins, P-FAK/FAK, and P-Pyk2/Pyk2 (P<0.01), which were all lowered in the treatment group (P<0.01). Correlation analysis suggested that the expressions of FAK mRNA, FAK, pFAK, Pyk2 mRNA and pPyk2/Pyk2 were positively correlated with proteinuria (r=0.819, 0.750, 0.838, 0.762, 0.934, respectively, P<0.01). CONCLUSIONS: Adriamycin increases phosphorylated FAK and Pyk2 expressions to mediate kidney injury in rats. Prednisone inhibits Pyk2 and FAK activation, decreases proteinuria, and alleviates podocyte lesions to protect the glomerular filtration barrier.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PTK2Humannephritis treatmentISOPtk2 (Rattus norvegicus) RGD 
PTK2BHumannephritis treatmentISOPtk2b (Rattus norvegicus) RGD 
Ptk2Ratnephritis treatmentIDA  RGD 
Ptk2Mousenephritis treatmentISOPtk2 (Rattus norvegicus) RGD 
Ptk2bRatnephritis treatmentIDA  RGD 
Ptk2bMousenephritis treatmentISOPtk2b (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptk2  (protein tyrosine kinase 2)
Ptk2b  (protein tyrosine kinase 2 beta)

Genes (Mus musculus)
Ptk2  (PTK2 protein tyrosine kinase 2)
Ptk2b  (PTK2 protein tyrosine kinase 2 beta)

Genes (Homo sapiens)
PTK2  (protein tyrosine kinase 2)
PTK2B  (protein tyrosine kinase 2 beta)


Additional Information