RGD Reference Report - Cellular function of RhoGDI-alpha mediates the cycling of Rac1 and the regulation of pancreatic beta cell death. - Rat Genome Database

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Cellular function of RhoGDI-alpha mediates the cycling of Rac1 and the regulation of pancreatic beta cell death.

Authors: Cho, Y  Huh, KH  Park, YJ  Do, JH  Joo, DJ  Kim, MS  Kim, YS 
Citation: Cho Y, etal., Transplant Proc. 2012 May;44(4):1083-5. doi: 10.1016/j.transproceed.2012.03.021.
RGD ID: 9684983
Pubmed: PMID:22564631   (View Abstract at PubMed)
DOI: DOI:10.1016/j.transproceed.2012.03.021   (Journal Full-text)

Mycophenolic acid (MPA) is an immunosuppressive agent that is widely used in clinical therapy, including pancreas and islet transplantation. Previously, we showed that MPA induces significant apoptosis of insulin-secreting cells by downregulating RhoGDI-alpha and increasing JNK expression. In this study, we investigated Rac1 directly associated with RhoGDI-alpha during MPA-induced apoptosis in INS-1E cells (an insulin-secreting cell line). Cells were treated with MPA for 24 and 36 hours. Immunoprecipitation was used to examine physical interactions between RhoGDI-alpha and Rac1. Activation and immunoprecipitation assays showed expressions of Rac1 and RhoGDI-alpha to be directly correlated. Rac1 binding to RhoGDI-alpha decreased after MPA treatment, and Rac1 was induced and subsequently activated by MPA. We concluded that this novel RhoGDI-alpha/Rac1/JNK pathway induced apoptosis of transplanted islet cells after MPA treatment.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ArhgdiaRatprotein binding  IPIRac1 (Rattus norvegicus) RGD 
Rac1RatRho GDP-dissociation inhibitor binding  IPIArhgdia (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arhgdia  (Rho GDP dissociation inhibitor alpha)
Rac1  (Rac family small GTPase 1)


Additional Information