RGD Reference Report - Identification of genes differentially expressed in mouse fetuses from streptozotocin-induced diabetic pregnancy by cDNA subtraction. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Identification of genes differentially expressed in mouse fetuses from streptozotocin-induced diabetic pregnancy by cDNA subtraction.

Authors: Sato, N  Sugimura, Y  Hayashi, Y  Murase, T  Kanou, Y  Kikkawa, F  Murata, Y 
Citation: Sato N, etal., Endocr J. 2008 May;55(2):317-23. Epub 2008 Mar 7.
RGD ID: 9586066
Pubmed: (View Article at PubMed) PMID:18323671

Epidemiological studies have shown that the risks of fetal malformation such as neural tube defects increase in diabetic pregnancy. To explore the mechanism of fetal malformation induced by diabetes, cDNA subtraction using mouse embryos (E9.5) of diabetic dams and those of controls was performed to identify differentially expressed genes. The expression level of genes identified by cDNA subtraction was further verified by quantitative RT-PCR using E8.5 embryos, and differential expression of 4 genes, Brcc3, Commd3, Ddx1, and SET was confirmed. We also analyzed the expression level of neural tube defect-related genes, and found that Folbp1, EphrinA5 and Sox10 were differentially expressed. Altered expression of these genes mostly persisted throughout the later stages of the development (E10.5-14.5). Hierarchical clustering analysis showed correlation between expression levels of these genes, suggesting that these genes cooperatively play a role in embryonic development. Our results suggest that an altered gene expression profile in embryos underlies the development of congenital malformation in diabetic pregnancies.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Brcc3  (BRCA1/BRCA2-containing complex, subunit 3)

Genes (Mus musculus)
Brcc3  (BRCA1/BRCA2-containing complex, subunit 3)

Genes (Homo sapiens)
BRCC3  (BRCA1/BRCA2-containing complex subunit 3)


Additional Information