RGD Reference Report - Changes in conjunctival clusterin expression in severe ocular surface disease. - Rat Genome Database

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Changes in conjunctival clusterin expression in severe ocular surface disease.

Authors: Nakamura, T  Nishida, K  Dota, A  Kinoshita, S 
Citation: Nakamura T, etal., Invest Ophthalmol Vis Sci. 2002 Jun;43(6):1702-7.
RGD ID: 8699507
Pubmed: PMID:12036968   (View Abstract at PubMed)

PURPOSE: Clusterin is a unique gene transcript in the human ocular surface epithelia and is synthesized by and localized in mucosal epithelia in general. It is not present, however, in keratinized epithelia, such as epidermis. In severe ocular surface disease, pathologic keratinization (squamous metaplasia) of the ordinarily nonkeratinized corneal and conjunctival mucosal epithelia results in severe visual loss. In the current study, the expression of clusterin was examined in conjunctivalized corneas with severe ocular surface disease. METHODS: We examined conjunctiva covering cornea in eight eyes with ocular surface disease (Stevens-Johnson syndrome and ocular cicatricial pemphigoid) in which pathologic keratinization was present. Normal conjunctiva from four age-matched individuals served as the control. Semiquantitative RT-PCR was used to investigate expression of the clusterin gene. Immunohistochemistry was used to study the distribution of clusterin protein. RESULTS: The level of clusterin mRNA was significantly lower than normal in the diseased ocular surfaces. Clusterin protein was also markedly decreased in keratinized conjunctiva compared with that in normal eyes. CONCLUSIONS: Clusterin expression is markedly reduced in the pathologic, keratinized ocular surface epithelium, suggesting its importance in maintaining the ocular surface epithelium as a nonkeratinizing epithelium.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CLUHumancicatricial pemphigoid  IEP mRNA:decreased expression:conjunctivaRGD 
CluRatcicatricial pemphigoid  ISOCLU (Homo sapiens)mRNA:decreased expression:conjunctivaRGD 
CluMousecicatricial pemphigoid  ISOCLU (Homo sapiens)mRNA:decreased expression:conjunctivaRGD 
CLUHumanStevens-Johnson syndrome  IEP mRNA:decreased expression:conjunctivaRGD 
CluRatStevens-Johnson syndrome  ISOCLU (Homo sapiens)mRNA:decreased expression:conjunctivaRGD 
CluMouseStevens-Johnson syndrome  ISOCLU (Homo sapiens)mRNA:decreased expression:conjunctivaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Clu  (clusterin)

Genes (Mus musculus)
Clu  (clusterin)

Genes (Homo sapiens)
CLU  (clusterin)


Additional Information