RGD Reference Report - Branched-chain amino acid-enriched nutrients stimulate antioxidant DNA repair in a rat model of liver injury induced by carbon tetrachloride. - Rat Genome Database

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Branched-chain amino acid-enriched nutrients stimulate antioxidant DNA repair in a rat model of liver injury induced by carbon tetrachloride.

Authors: Ichikawa, K  Okabayashi, T  Shima, Y  Iiyama, T  Takezaki, Y  Munekage, M  Namikawa, T  Sugimoto, T  Kobayashi, M  Mimura, T  Hanazaki, K 
Citation: Ichikawa K, etal., Mol Biol Rep. 2012 Dec;39(12):10803-10. doi: 10.1007/s11033-012-1974-4. Epub 2012 Oct 9.
RGD ID: 8657403
Pubmed: PMID:23053977   (View Abstract at PubMed)
DOI: DOI:10.1007/s11033-012-1974-4   (Journal Full-text)

Oxidative stress (OS) plays an important role in the progression of chronic liver disease including organ injury and hypoalbuminemia. Long-term oral supplementation with branched-chain amino acids (BCAAs) can inhibit liver dysfunction but their role in the prevention of liver fibrosis and injury to the liver is unclear. The aim of this study was to assess how BCAAs preserve liver function from OS. To investigate how BCAAs specifically prevent OS, we evaluated the effect of oral supplementation with BCAAs on OS using a rat liver cirrhosis model. Liver cirrhosis was induced in ten male Sprague-Dawley rats by administering carbon tetrachloride for 12 weeks. Five of the ten carbon tetrachloride-treated rats were assigned to a control group and five to a BCAA group. BCAA-supplementation significantly preserved plasma albumin concentrations and significantly inhibited the occurrence of organ injury as determined by blood chemistry analysis. Hepatic expression of OGG1 mRNA was increased in the BCAA group compared to the control group. In the BCAA group, increased hepatic levels of OGG1 protein were found by western blot. On the other hand, the number of 8-OHdG-positive cells was significantly higher in liver sections taken 1 month after carbon tetrachloride treatment. Furthermore, OGG1-positive cells were significantly increased in the hepatocytes around the central vein. BCAA was found to reduce OS, which could possibly lead to a decrease in the occurrence of hypoalbuminemia and organ injury. Our results indicate that BCAA-enriched nutrients stimulate antioxidant DNA repair in a rat model of liver injury induced by carbon tetrachloride.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
OGG1HumanExperimental Liver Cirrhosis treatmentISOOgg1 (Rattus norvegicus) RGD 
Ogg1RatExperimental Liver Cirrhosis treatmentIEP  RGD 
Ogg1MouseExperimental Liver Cirrhosis treatmentISOOgg1 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ogg1  (8-oxoguanine DNA glycosylase)

Genes (Mus musculus)
Ogg1  (8-oxoguanine DNA-glycosylase 1)

Genes (Homo sapiens)
OGG1  (8-oxoguanine DNA glycosylase)


Additional Information