RGD Reference Report - Mitochondrial carnitine palmitoyltransferase 1a (CPT1a) is part of an outer membrane fatty acid transfer complex. - Rat Genome Database

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Mitochondrial carnitine palmitoyltransferase 1a (CPT1a) is part of an outer membrane fatty acid transfer complex.

Authors: Lee, K  Kerner, J  Hoppel, CL 
Citation: Lee K, etal., J Biol Chem. 2011 Jul 22;286(29):25655-62. doi: 10.1074/jbc.M111.228692. Epub 2011 May 26.
RGD ID: 8554441
Pubmed: PMID:21622568   (View Abstract at PubMed)
PMCID: PMC3138250   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M111.228692   (Journal Full-text)

CPT1a (carnitine palmitoyltransferase 1a) in the liver mitochondrial outer membrane (MOM) catalyzes the primary regulated step in overall mitochondrial fatty acid oxidation. It has been suggested that the fundamental unit of CPT1a exists as a trimer, which, under native conditions, could form a dimer of the trimers, creating a hexamer channel for acylcarnitine translocation. To examine the state of CPT1a in the MOM, we employed a combined approach of sizing by mass and isolation using an immunological method. Blue native electrophoresis followed by detection with immunoblotting and mass spectrometry identified large molecular mass complexes that contained not only CPT1a but also long chain acyl-CoA synthetase (ACSL) and the voltage-dependent anion channel (VDAC). Immunoprecipitation with antisera against the proteins revealed a strong interaction between the three proteins. Immobilized CPT1a-specific antibodies immunocaptured not only CPT1a but also ACSL and VDAC, further strengthening findings with blue native electrophoresis and immunoprecipitation. This study shows strong protein-protein interaction between CPT1a, ACSL, and VDAC. We propose that this complex transfers activated fatty acids through the MOM.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitochondrial outer membrane located_inIDA 8554441PMID:21622568UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Cpt1a  (carnitine palmitoyltransferase 1A)


Additional Information