RGD Reference Report - Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2. - Rat Genome Database

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Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2.

Authors: Park, HW  Jung, H  Choi, KH  Baik, JH  Rhim, H 
Citation: Park HW, etal., FEBS Lett. 2010 Aug 4;584(15):3317-22. doi: 10.1016/j.febslet.2010.07.014. Epub 2010 Jul 11.
RGD ID: 8554172
Pubmed: PMID:20627102   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2010.07.014   (Journal Full-text)

Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.




Molecular Function

  
Object Symbol
Species
Term
Qualifier
Evidence
With
Notes
Source
Original Reference(s)
Cacna1dRatprotein binding enablesIPIUniProtKB:O88871PMID:20627102IntAct 
Gabbr2Ratprotein binding enablesIPIUniProtKB:P27732PMID:20627102IntAct 


Genes (Rattus norvegicus)
Cacna1d  (calcium voltage-gated channel subunit alpha1 D) Gabbr2  (gamma-aminobutyric acid type B receptor subunit 2)