RGD Reference Report - Multiplex immunoassay analysis of cytokines in idiopathic inflammatory myopathy. - Rat Genome Database

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Multiplex immunoassay analysis of cytokines in idiopathic inflammatory myopathy.

Authors: Baird, Geoffrey S  Montine, Thomas J 
Citation: Baird GS and Montine TJ, Arch Pathol Lab Med. 2008 Feb;132(2):232-8. doi: 10.1043/1543-2165(2008)132[232:MIAOCI]2.0.CO;2.
RGD ID: 8549795
Pubmed: PMID:18251582   (View Abstract at PubMed)
DOI: DOI:10.1043/1543-2165(2008)132[232:MIAOCI]2.0.CO;2   (Journal Full-text)


CONTEXT: Idiopathic inflammatory myopathies (IIMs), including dermatomyositis, polymyositis, and inclusion-body myositis, can be difficult to diagnose.
OBJECTIVE: To determine if a multiplex immunoassay for markers of inflammation in muscle homogenates correlates with a diagnosis of IIM.
DESIGN: Frozen archived muscle biopsy specimens from 30 patients with IIM and 34 patients without IIM were homogenized and analyzed for cytokine content with a multiplex microbead-based immunoassay system. Analyte concentrations were normalized to total lysate protein concentration prior to comparison.
RESULTS: Two cytokines, interleukin 1ra and monocyte chemoattractant protein 1, and 1 soluble adhesion molecule, intracellular adhesion molecule 1, were found at significantly greater concentrations in muscle samples from patients with IIM. Intracellular adhesion molecule 1 levels alone were 83% sensitive and 91% specific for IIM at a cutoff of 1240 pg/mg muscle protein.
CONCLUSIONS: Immunoassays for selected inflammatory markers can serve in conjunction with histopathologic analysis as sensitive and specific tools for the diagnosis of IIM.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL1RNHumanmyositis  IEP protein:increased expression:skeletal muscle (human)RGD 
Il1rnRatmyositis  ISOIL1RN (Homo sapiens)protein:increased expression:skeletal muscle (human)RGD 
Il1rnMousemyositis  ISOIL1RN (Homo sapiens)protein:increased expression:skeletal muscle (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il1rn  (interleukin 1 receptor antagonist)

Genes (Mus musculus)
Il1rn  (interleukin 1 receptor antagonist)

Genes (Homo sapiens)
IL1RN  (interleukin 1 receptor antagonist)


Additional Information