RGD Reference Report - Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3. - Rat Genome Database

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Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3.

Authors: Sinha, S  Maity, SN  Lu, J  De Crombrugghe, B 
Citation: Sinha S, etal., Proc Natl Acad Sci U S A 1995 Feb 28;92(5):1624-8.
RGD ID: 729287
Pubmed: PMID:7878029   (View Abstract at PubMed)
PMCID: PMC42572   (View Article at PubMed Central)

The CCAAT binding factor CBF is a heteromeric transcription factor, which binds to functional CCAAT motifs in many eukaryotic promoters. cDNAs for the A and B subunits of CBF (CBF-A and CBF-B) and for their yeast homologues HAP3 and HAP2 have been previously isolated, but the purified recombinant CBF-A and CBF-B together are unable to bind to CCAAT motifs in DNA. Here we report the isolation of a cDNA coding for rat CBF-C, demonstrate that recombinant CBF-C is required together with CBF-A and CBF-B to form a CBF-DNA complex, and show that CBF-C is present in this protein-DNA complex together with the other two subunits. We further show that CBF-C allows formation of a complex between the purified recombinant yeast HAP2 and HAP3 polypeptides and a CCAAT-containing DNA and is present in this complex, implying the existence of a CBF-C homologue in yeast. We show that CBF-A and CBF-C interact with each other to form a CBF-A-CBF-C complex and that CBF-B does not interact with CBF-A or CBF-C individually but that it associates with the CBF-A-CBF-C complex. Our results indicate that CBF is a unique evolutionarily conserved DNA binding protein.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NfyaRattranscription by RNA polymerase II  ICGO:0016602 RGD 
NfybRattranscription by RNA polymerase II  ICGO:0016602 RGD 
NfycRattranscription by RNA polymerase II  ICGO:0016602 RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NfyaRatCCAAT-binding factor complex  IDA  RGD 
NfybRatCCAAT-binding factor complex  IDA  RGD 
NfycRatCCAAT-binding factor complex  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NfybRatprotein-containing complex binding  IDA  RGD 
NfyaRattranscription factor binding  IPINfyc (Rattus norvegicus) RGD 
NfycRattranscription factor binding  IPINfya (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nfya  (nuclear transcription factor Y subunit alpha)
Nfyb  (nuclear transcription factor Y subunit beta)
Nfyc  (nuclear transcription factor Y subunit gamma)


Additional Information