RGD Reference Report - Differential distribution of fibroblast growth factor (FGF)-7 and FGF-10 in L-arginine-induced acute pancreatitis. - Rat Genome Database

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Differential distribution of fibroblast growth factor (FGF)-7 and FGF-10 in L-arginine-induced acute pancreatitis.

Authors: Ishiwata, T  Naito, Z  Lu, YP  Kawahara, K  Fujii, T  Kawamoto, Y  Teduka, K  Sugisaki, Y 
Citation: Ishiwata T, etal., Exp Mol Pathol 2002 Dec;73(3):181-90.
RGD ID: 727503
Pubmed: PMID:12565793   (View Abstract at PubMed)

The regenerative process of the pancreas after acute pancreatitis (AP) is characterized by acinar and ductal cell proliferation with synthesis and transient deposition of extracellular matrices. Various growth factors were reported to be highly expressed in AP, but their regulation has not yet been clarified. Fibroblast growth factor (FGF)-7, also known as keratinocyte growth factor (KGF), and FGF-10 are members of the FGF family and show high structural homology and similar biological characteristics. Both are mainly synthesized by mesenchymal cells and stimulate epithelial cells via KGF receptor (KGFR) which is a splice variant of FGFR-2. In the present study, we attempted to immunohistochemically determine the localization of FGF-7 and FGF-10 in pancreatic tissues of an L-arginine-induced rat pancreatitis model. Furthermore, highly specific KGFR antibodies were prepared and used for Western blot analysis and immunohistochemistry. In the normal pancreas, FGF-7 was localized in alpha cells of islets, but FGF-10 was not detected. KGFR was also localized in islet cells, ductal cells, and centroacinar cells in the normal pancreas. In the pancreatic tissues of rats with L-arginine-induced pancreatitis, FGF-7 was localized in alpha cells, whereas FGF-10 was expressed in vascular smooth muscle cells (VSMCs). KGFR was not expressed in centroacinar cells and its level decreased after L-arginine treatment. However, KGFR was detected instead in some acinar cells and VSMCs in addition to islet cells. These findings suggest that FGF-7 and FGF-10 contribute to the regeneration and differentiation of acinar cells and angiogenesis in AP through KGFR.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Fgf10Ratangiogenesis  IEP  RGD 
Fgf10Ratcell differentiation  IEP  RGD 
Fgf10Ratfibroblast growth factor receptor signaling pathway  TAS  RGD 
Fgf10Rattissue regeneration  IEP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Fgf10Ratfibroblast growth factor receptor binding  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fgf10  (fibroblast growth factor 10)

Objects referenced in this article
Gene Fgf7 fibroblast growth factor 7 Rattus norvegicus

Additional Information